Phospholipid Composition and Metabolism of Micrococcus denitrificans
Phospholipid Composition and Metabolism of Micrococcus denitrificans
复制标题
脱氮微球菌的磷脂组成和代谢
作者：
B. Wilkinson;M. Morman;D. White
磷脂酰胆碱（PC）是一种主要的磷脂（30.9%）的微球菌的磷脂组成是不寻常的。其他磷脂为磷脂酰甘油（PG，52.4%）、磷脂酰乙醇胺（PE，5.8%）、未知磷脂（5.3%）、心磷脂（CL，3.2%）、磷脂酰二甲基乙醇胺（PDME，0.9%）、磷脂酰单甲基乙醇胺（PMME，0.6%）、磷脂酰丝氨酸（PS，0.5%）和磷脂酸（0.4%）。~（32）P掺入动力学表明，PC是由PE的连续甲基化反应形成的。脉冲追逐实验与脉冲的32 P或乙酸-1-14 C指数增长的细胞显示损失的同位素PMME，PDME，PS，CL与双相动力学表明相同类型的多个池的这些脂质中提出的其他细菌。主要磷脂PC、PG和PE在这些条件下代谢稳定。从复合脂质中分离出的脂肪酸也是不寻常的，它是7种脂肪酸的简单混合物，其中油酸占总量的86%。很少的游离脂肪酸和不可提取的脂肪酸与细胞壁或膜被发现。
The phospholipid composition of Micrococcus denitrificans was unusual in that phosphatidyl choline (PC) was a major phospholipid (30.9%). Other phospholipids were phosphatidyl glycerol (PG, 52.4%), phosphatidyl ethanolamine (PE, 5.8%), an unknown phospholipid (5.3%), cardiolipin (CL, 3.2%), phosphatidyl dimethylethanolamine (PDME, 0.9%), phosphatidyl monomethylethanolamine (PMME, 0.6%), phosphatidyl serine (PS, 0.5%), and phosphatidic acid (0.4%). Kinetics of 32P incorporation suggested that PC was formed by the successive methylations of PE. Pulse-chase experiments with pulses of 32P or acetate-1-14C to exponentially growing cells showed loss of isotopes from PMME, PDME, PS, and CL with biphasic kinetics suggesting the same type of multiple pools of these lipids as proposed in other bacteria. The major phospholipids, PC, PG, and PE, were metabolically stable under these conditions. The fatty acids isolated from the complex lipids were also unusual in being a simple mixture of seven fatty acids with oleic acid representing 86% of the total. Few free fatty acids and no non-extractable fatty acids associated with the cell wall or membrane were found.