Correction of developmental abnormalities resulting from localized ultra-violet irradiation of an amphibian egg. 1.
Correction of developmental abnormalities resulting from localized ultra-violet irradiation of an amphibian egg. 1.
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纠正两栖动物卵局部紫外线照射导致的发育异常。
DOI：
10.1002/jez.1401890213
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
H. Chung
中科院分区：
文献类型：
--
作者：
G. Malacinski;C. Allis;H. Chung
未切割的 R.pipienseggs 的植物半球的紫外线照射导致神经形态发生中特征性缺陷的发展。如果使用紫外线，则可以防止这些缺陷的发生。受辐射的胚胎要么（1）在低温下孵育（冷冻复归），（2）显微注射卵母细胞匀浆，或（3）通过在原肠胚形成时用正常（未经紫外线辐射）胚胎的唇部替换其背唇来进行手术操作。
Ultra‐violet irradiation of the vegetal hemisphere of uncleavedR.pipienseggs results in the development of characteristic defects in neural morphogenesis. These defects can be prevented from occurring if U.V. irradiated embryos are either (1) incubated at low temperature (cryoreversion), (2) microinjected with oocyte homogenate, or (3) surgically manipulated by replacing their dorsal lip with the lip of a normal (non U.V. irradiated ) embryo at the time of gastrulation.