Structure of a packaging-defective mutant of minute virus of mice indicates that the genome is packaged via a pore at a 5-fold axis.
Structure of a packaging-defective mutant of minute virus of mice indicates that the genome is packaged via a pore at a 5-fold axis.
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小鼠微小病毒包装缺陷突变体的结构表明，基因组是通过5倍轴上的孔进行包装的。
DOI：
10.1128/jvi.02598-10
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发表时间：
2011
影响因子：
5.4
通讯作者：
Tattersall,Peter
中科院分区：
文献类型：
--
作者：
Plevka,Pavel;Hafenstein,Susan;Li,Lei;D'AbrgamoJr,Anthony;Cotmore,SusanF;Rossmann,MichaelG;Tattersall,Peter
小鼠细小病毒（MVM）将其线性单链DNA基因组的单个拷贝包装到预先形成的衣壳中，这一过程可能是由病毒编码的解旋酶驱动的。细小病毒有一个大致圆柱形的孔，围绕着12个5重顶点。穿透病毒体外壳的孔是由10条反向平行的β链并置产生的，其中两条来自5倍相关的衣壳蛋白。在172位的亮氨酸的氨基酸相关侧链形成的通道中存在瓶颈。我们在这里报告的X-射线晶体结构的粒子产生的亮氨酸到色氨酸突变的位置172和分析其生化特性。突变体衣壳的5倍通道被阻断，并且颗粒不能包装DNA，这强烈表明5倍孔是基因组进入的包装门户。
The parvovirus minute virus of mice (MVM) packages a single copy of its linear single-stranded DNA genome into preformed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the 12 5-fold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of 10 antiparallel β-strands, two from each of the 5-fold-related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related side chains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine-to-tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid had its 5-fold channel blocked, and the particles were unable to package DNA, strongly suggesting that the 5-fold pore is the packaging portal for genome entry.