Factors Influencing Microbiological Biodiversity of Human Foot Skin
Factors Influencing Microbiological Biodiversity of Human Foot Skin
复制标题
影响人类足部皮肤微生物生物多样性的因素
DOI：
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发表时间：
2019
影响因子：
--
通讯作者：
B. Gutarowska
中科院分区：
文献类型：
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作者：
Aleksandra Steglińska;Anita Jachowicz;Justyna Szulc;Justyna W. Adamiak;Anna Otlewska;Katarzyna Pielech;B. Gutarowska
该研究的目的是分析人类足部皮肤的微生物多样性，包括年龄、性别、洗脚频率和体力活动等因素，并选择在设计用于袜子和鞋垫生产的具有抗菌性能的纺织材料时要考虑的指标物种。该实验针对 40 人进行。使用依赖培养的方法测定微生物的数量。使用培养物确定生物多样性，然后根据 16S rRNA 基因测序（细菌）、ITS 区域（真菌）或使用 Illumina 下一代测序（在一组八个选定个体中）进行遗传鉴定。女性足部细菌总数平均为1.0×106 CFU/cm2，与男性足部细菌总数（平均1.2×105 CFU/cm2）无统计学差异。在大多数情况下，细菌的数量随着年龄的增长和体力活动频率的增加而减少。细菌数量随着洗脚频率的减少而增加；然而，组间发现了统计学上的显着差异。各组之间真菌的数量没有显着差异。厚壁菌门、变形菌门和放线菌门的细菌构成了足部皮肤的主要微生物。真菌中以子囊菌门和担子菌门为主。特定物种的存在因上述因素而异。已确定的两个物种被归类为病原体（淡黄奈瑟菌和烟曲霉）。这些发现表明，有必要扩大纺织品的卫生性能测试微生物清单。
The aim of the study was to analyze the microbiological biodiversity of human foot skin with respect to factors such as age, gender, frequency of foot washing and physical activity, and to select indicator species to be considered when designing textile materials with antimicrobial properties used for sock and insole production. The experiment was carried out on a group of 40 people. The number of microorganisms was determined using culture-dependent methods. Biodiversity was determined using culture followed by genetic identification based on 16S rRNA gene sequencing (bacteria), ITS region (fungi), or using Illumina next-generation sequencing (in a group of eight selected individuals). The total bacterial number on women’s feet was on average 1.0 × 106 CFU/cm2, and was not statistically significantly different than that of men’s feet (mean 1.2 × 105 CFU/cm2). The number of bacteria, in most cases, decreased with age and with increased frequency of physical activity. The number of bacteria increased with diminishing feet-washing frequency; however, statistically significant differences were found between groups. The number of fungi was not significantly different amongst groups. Bacteria belonging to the phyla Firmicutes, Proteobacteria and Actinobacteria constituted the main microorganisms of the foot skin. Ascomycota and Basidiomycota predominated amongst the fungi. The presence of specific species varied in groups depending on the factors mentioned above. Two of the species identified were classified as pathogens (Neisseria flavescens and Aspergillus fumigatus). These findings suggest that it is necessary to extend the list of microorganisms tested on textiles with respect to hygienic properties.
我们皮肤上的生物：皮肤微生物组的生态学、基因组学和治疗机会。
DOI：
10.1016/j.ddmec.2012.12.003
发表时间：
2013
期刊：
Drug discovery today. Disease mechanisms
影响因子：
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作者：
Scharschmidt,TiffanyC;Fischbach,MichaelA
通讯作者：
Fischbach,MichaelA