In vitro cultivation of Babesia bigemina.
In vitro cultivation of Babesia bigemina.
复制标题
二联巴贝虫的体外培养。
DOI：
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发表时间：
1985
影响因子：
1
通讯作者：
C. Carson
中科院分区：
文献类型：
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作者：
Vega Ca;G. Buening;T. Green;C. Carson
从感染的小牛中分离出一株二联巴氏杆菌，并在体外繁殖。培养条件包括在特殊溶液中洗涤感染和正常牛红细胞，以及在5%CO2、2%O2、93%N2气氛中使用5%至10%（v/v）的红细胞悬液在培养基199（含20%至50%新鲜正常牛血清）中4 mm的深度。在体外培养36天和9次传代培养后，将培养的微生物接种到易感小牛体内。该小牛出现疾病的临床体征，并在用1%台盼蓝溶液处理后恢复。还从第二头小牛中重新分离出该菌株。原始分离物在体外连续培养99天以上。
A strain of Babesia bigemina was isolated from an infected calf and propagated in vitro. Culture conditions included washing of infected and normal bovine erythrocytes in a special solution, and the use of a 5% to 10% (v/v) erythrocyte suspension in medium 199 (with 20% to 50% fresh normal bovine serum) at a depth of 4 mm in a 5% CO2, 2% O2, 93% N2 atmosphere. After 36 days in vitro and 9 subcultures, the cultured organism was inoculated into a susceptible calf. This calf developed clinical signs of disease and recovered when treated with 1% trypan blue solution. The strain was also reisolated from the second calf. The original isolate had been maintained in continuous in vitro cultivation for more than 99 days.