Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages
Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages
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DOI：
10.1111/j.1742-4658.2006.05552.x
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发表时间：
2006-12-01
期刊：
影响因子：
5.4
通讯作者：
Botham, Kathleen M.
中科院分区：
文献类型：
--
作者：
De Pascale, Clara;Avella, Michael;Botham, Kathleen M.
研究了乳糜微粒残余物样颗粒（CRLPs）脂肪酸组成对巨噬细胞摄取和诱导脂质积累的影响。使用分别富含棕榈、橄榄、玉米或鱼油中饱和、单不饱和、n-6或n-3多不饱和脂肪酸的含有三酰基甘油的CRLPs，以及来自人类单核细胞系THP-1的巨噬细胞。在与CRLPs孵养5、24和48小时后，测量细胞中的脂质积累（三酰甘油和胆固醇），并使用[h -3]三油苷放射性标记的CRLPs测定24小时内的摄取情况。与其他三种类型的颗粒相比，棕榈CRLPs在巨噬细胞中的总脂质积累显著增加。这主要是由于甘油三酯浓度的增加，而胆固醇浓度的变化没有达到显著性。与橄榄、玉米或鱼类CRLPs孵育后，脂质积累无显著差异。棕榈和橄榄的crlp被细胞吸收的速度相似，比玉米和鱼类的crlp要快得多。这些发现表明，与富含n-6或n-3多不饱和脂肪酸的CRLPs相比，富含饱和或单不饱和脂肪酸的CRLPs被巨噬细胞吸收的速度更快，并且在富含饱和脂肪酸的颗粒中，更快的吸收速度导致更多的脂质积累，而在富含单不饱和脂肪酸的颗粒中则不然。因此，乳糜微粒残留物中携带的膳食饱和脂肪酸可能增强其诱导巨噬细胞泡沫细胞形成的倾向。
The influence of the fatty acid composition of chylomicron remnant-like particles (CRLPs) on their uptake and induction of lipid accumulation in macrophages was studied. CRLPs containing triacylglycerol enriched in saturated, monounsaturated, n-6 or n-3 polyunsaturated fatty acids derived from palm, olive, corn or fish oil, respectively, and macrophages derived from the human monocyte cell line THP-1 were used. Lipid accumulation (triacylglycerol and cholesterol) in the cells was measured after incubation with CRLPs for 5, 24 and 48 h, and uptake over 24 h was determined using CRLPs radiolabelled with [H-3]triolein. Total lipid accumulation in the macrophages was significantly greater with palm CRLPs than with the other three types of particle. This was mainly due to increased triacylglycerol concentrations, whereas changes in cholesterol concentrations did not reach significance. There were no significant differences in lipid accumulation after incubation with olive, corn or fish CRLPs. Palm and olive CRLPs were taken up by the cells at a similar rate, which was considerably faster than that observed with corn and fish CRLPs. These findings demonstrate that CRLPs enriched in saturated or monounsaturated fatty acids are taken up more rapidly by macrophages than those enriched in n-6 or n-3 polunsaturated fatty acids, and that the faster uptake rate results in greater lipid accumulation in the case of saturated fatty acid-rich particles, but not monounsaturated fatty acid-rich particles. Thus, dietary saturated fatty acids carried in chylomicron remnants may enhance their propensity to induce macrophage foam cell formation.