Crawling motility of Treponema denticola modulated by outer sheath protein
Crawling motility of Treponema denticola modulated by outer sheath protein
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DOI：
10.1111/1348-0421.12940
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发表时间：
2021-11-15
影响因子：
2.6
通讯作者：
Ishihara, Kazuyuki
中科院分区：
文献类型：
--
作者：
Kokubu, Eitoyo;Kikuchi, Yuichiro;Ishihara, Kazuyuki
齿密螺旋体是一种螺旋状可移动微生物，是慢性牙周炎的主要病原体。主要表面蛋白(MSP)和牙菌素是位于外鞘的齿状毛滴虫的毒力因子。齿纹夜蛾的运动性与其在寄主组织上的定植和入侵密切相关。外鞘位于环境与齿状毛滴虫的交界处，其成分也可能通过与细胞外物质的相互作用而促进其运动。这项研究的目的是通过使用MSP缺陷株和牙菌素缺陷株来研究MSP或牙菌素是否对齿状毛滴虫在固体表面上的移动(称为爬行)起作用。通过测量琼脂平板上的菌落大小来分析菌落的运动能力，用暗视野显微镜分析菌落的速度。突变菌株的菌落面积小于野生型菌株。突变菌株的爬行速度低于野生型菌株，其中牙菌素缺陷型菌株的爬行速度最低。此外，牙菌素突变体的一圈爬行距离与原生质柱间距(一个衡量爬行效率的指标)的比值显著低于野生型和MSP突变体。综上所述，这些结果表明牙菌素促进了齿纹夜蛾的爬行依赖的表面传播。
Treponema denticola, a helically shaped motile microorganism, is a major pathogen of chronic periodontitis. Major surface protein (Msp) and dentilisin are virulence factors of T. denticola that are located on the outer sheath. The motility of T. denticola is deeply involved in colonization on and invasion into the host tissue. The outer sheath is located at the interface between the environment and T. denticola, and its components may also contribute to its motility via interaction with the materials outside the cells. The study aimed to clarify whether Msp or dentilisin contributes to the motility of T. denticola on solid surfaces, termed crawling, by investigating their effects using Msp-deficient and dentilisin-deficient T. denticola strains. Motility was analyzed by measuring the colony size in agar plates and velocity was analyzed using dark-field microscopy. The colony area of the mutant strains was smaller than that of the wild-type strain. The crawling velocity of the mutant strains was lower than that of the wild-type strain, with the lowest velocity observed in the dentilisin-deficient strain. Additionally, the ratio of the crawling distance by one revolution to the protoplasmic cylinder pitch (an indicator of the crawling efficiency) in the dentilisin mutant was significantly lower than that in the wild type strain and the Msp mutant. Together, these results indicate that dentilisin facilitates the crawling-dependent surface spreading of T. denticola.