Ontogenesis of the vitamin D receptor in rat heart
Ontogenesis of the vitamin D receptor in rat heart
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DOI：
10.1007/s00418-002-0413-3
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发表时间：
2002-06-01
影响因子：
2.3
通讯作者：
Pérez-Fernández, R
中科院分区：
文献类型：
--
作者：
Fraga, C;Blanco, M;Pérez-Fernández, R
1,25-二羟基维生素D-3通过其受体（vitamin D receptor； VDR）具有钙转运和细胞生长分化等重要生理作用。尽管VDR存在于多种细胞系以及包括大鼠和人类心脏在内的许多组织中，但没有关于大鼠生命不同阶段心脏中VDR存在的数据。本研究采用RT-PCR和免疫组织化学技术检测了VDR在胎儿（17、18和20妊娠日）、新生儿（4和8妊娠日）和成年大鼠心脏中的表达。免疫组化技术显示VDR蛋白定位于心肌纤维细胞核。此外，我们证明了VDR mRNA的表达在这些不同的发育时期发生变化，在胎龄20天和18天表现出显著差异。这些VDR表达的变化可能与心肌发育和/或细胞内心肌细胞钙稳态相关的其他参数有关。
1,25-Dihydroxyvitamin D-3 through its receptor (vitamin D receptor; VDR) has important physiological effects such as calcium transport and cell growth and differentiation. Although the VDR is present in a variety of cell lines as well as in numerous tissues, including rat and human heart, no data are available about the presence of VDR in heart at different steps of rat life. In this study we evaluated the VDR expression using RT-PCR and immunohistochemical techniques in fetal (17, 18 and 20 gestational days), neonatal (4 and 8 days) and adult rat heart. Immunohistochemical techniques showed the VDR protein localisation in the nuclei of cardiac muscle fibres. Also, we demonstrated that VDR mRNA expression is changing over these different periods of development, showing significant differences in 20 days versus 18 days of fetal age. These changes in VDR expression may be related to other parameters associated with the development of the cardiac muscle and/or intracellular cardiac cell calcium homeostasis.