Degeneration process of Lewy bodies in the brains of patients with dementia with Lewy bodies using α-synuclein-immunohistochemistry
Degeneration process of Lewy bodies in the brains of patients with dementia with Lewy bodies using α-synuclein-immunohistochemistry
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DOI：
10.1016/s0304-3940(00)01090-9
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发表时间：
2000-05-26
影响因子：
2.5
通讯作者：
Kosaka, K
中科院分区：
文献类型：
--
作者：
Iseki, E;Marui, W;Kosaka, K
应用α-突触核蛋白免疫组织化学方法研究了路易体痴呆患者脑内路易体的变性过程。细胞内LB，LB相关的神经突起和一些细胞外LB与抗α-突触核蛋白抗体免疫染色呈阳性。同心LB轴承神经元没有小胶质细胞参与，而退化的神经元与不明确的LB显示强烈的小胶质细胞参与。细胞外LB的晚期阶段是由松散的聚集体的丝状组件与丢失的α-突触核蛋白的免疫反应性和渗透星形胶质细胞的过程。这些结果表明，小胶质细胞参与了从退化的LB承载神经元到细胞外LB的阶段，而星形胶质细胞参与了细胞外LB的阶段。一些细胞内LB的抗C3 d和C4d抗体阳性，表明经典的补体途径在退化的LB承载神经元中被激活，诱导小胶质细胞活化和神经元死亡。(C)2000由Elsevier Science爱尔兰有限公司出版。保留所有权利。
We investigated the degeneration process of Lewy bodies (LB) in the brains of dementia with Lewy bodies, using alpha-synuclein-immunohistochemistry. Intracellular LB, LB-related neurites and some extracellular LB were positively immunostained with anti-alpha-synuclein antibodies. Concentric LB-bearing neurons had no microglial involvement, while degenerated neurons with ill-defined LB displayed intense microglial involvement. The late stage of extracellular LB were immunoelectron-microscopically composed of loose aggregates of filamentous components with lost alpha-synuclein-immunoreactivity and penetrated astroglial processes. These findings suggest that microglias are involved during the stages from degenerated LB-bearing neurons to extracellular LB, while astroglias are involved during the stage of extracellular LB, Some intracellular LB were positive for anti-C3d and -C4d antibodies, suggesting that the classical complement pathway is activated in degenerated LB-bearing neurons, inducing microglial activation and neuronal death. (C) 2000 Published by Elsevier Science Ireland Ltd. All rights reserved.