Co-occurrence patterns and assembly processes of microeukaryotic communities in an early-spring diatom bloom
Co-occurrence patterns and assembly processes of microeukaryotic communities in an early-spring diatom bloom
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早春硅藻华微真核生物群落的共现模式和组装过程
DOI：
10.1016/j.scitotenv.2019.134624
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发表时间：
2020-04-01
影响因子：
9.8
通讯作者：
Zhang, Demin
中科院分区：
文献类型：
--
作者：
Hou, Fanrong;Zhang, Huajun;Zhang, Demin
在有害藻华等强烈干扰的环境中，微真核生物群落组合物（MEC）的相互作用和组装过程很少被阐明。为了填补这一空白，我们使用 18S rRNA 基因扩增子测序分析了硅藻华引起的 MEC 变化。 MEC 主要以尾虫（平均相对丰度为 49.2%）、硅藻（25.5%）和甲藻（15.6%）为主。 MEC 在四花期发生显着变化（ANOSIM P < 0.01）。 pH、DO、硝酸盐和磷酸盐等环境因素以及细菌群落可以显着影响 MEC 的变化。共现网络分析揭示了微真核生物和细菌群落之间复杂的相互作用。共现网络模块中的大多数 OTU 都特定于一个特定的绽放阶段。基于系统发育的β-最近分类单元距离分析表明，随机过程主要主导微真核生物群落的初始和开花后阶段的组装。然而，花期中后期的微真核生物群落组装是由确定性过程驱动的。总之，随机过程和确定性过程在不同的绽放阶段都发挥着重要作用。这些发现可能会扩大目前对有害藻华频繁发生的富营养化水生生态系统中微真核动力学的组装机制和微生物相互作用的理解。 (C) 2019 Elsevier B.V. 保留所有权利。
The interaction and assembly processes of microeukaryotic community compositions (MECs) are rarely elucidated in environment with strong disturbance such as harmful algal blooms. To fill this gap, we analyzed changes of MECs induced by a diatom bloom using 18S rRNA gene amplicon sequencing. The MECs were mainly dominated by Cercozoa (average relative abundance, 49.2%), Diatom (25.5%) and Dinoflagellata (15.6%). MECs changed significantly (ANOSIM P < 0.01) in four-bloom stages. Environmental factors including pH, DO, nitrate and phosphate, together with bacterial communities could significantly influence the variation of MECs. Co-occurrence network analysis revealed a complex interaction between microeukaryotic and bacterial communities. Most OTUs in modules of the co-occurrence network were specific to one particular bloom stage. Phylogenetic based beta-nearest taxon distance analyses revealed that stochastic processes mainly dominated microeukaryotic community assembly in the initial and after-bloom stage. However, microeukaryotic community assembly in middle and late stage of the bloom were driven by deterministic processes. In conclusion, both stochastic and deterministic processes play important roles in distinct bloom stages. These findings may expand current understandings of assembly mechanisms and microbial interactions underlying microeukaryotic dynamics in eutrophic aquatic ecosystems where harmful algal blooms occurred frequently. (C) 2019 Elsevier B.V. All rights reserved.