Screening for estrogenic steroids in sheep and chicken by the application of enzyme-linked immunosorbent assay and a comparison with analysis by gas chromatography–mass spectrometry
Screening for estrogenic steroids in sheep and chicken by the application of enzyme-linked immunosorbent assay and a comparison with analysis by gas chromatography–mass spectrometry
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DOI：
10.1016/s0308-8146(98)00056-9
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发表时间：
1998-12
期刊：
影响因子：
8.8
通讯作者：
W. Sawaya;K. Lone;A. Husain;B. Dashti;S. Al-Zenki
中科院分区：
文献类型：
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作者：
W. Sawaya;K. Lone;A. Husain;B. Dashti;S. Al-Zenki
对从科威特国当地市场上出售的禽类（来自巴西、丹麦、法国和土耳其）中采集的146份羊尿和87份鸡肌肉样品进行了两种外源性雌激素化合物（即己烯雌酚（DES）和炔雌醇）残留物检测。采用酶联免疫吸附试验（ELISA）和气相色谱-质谱（GC-MS）对样品进行分析。结果表明，尿中DES和炔雌醇的含量分别为未检出（ND）~1.2和ND~0.90ppb。在鸡肌肉中，检测到的水平分别为ND至0.70和ND至0.30ppb，这表明根据国际组织的建议，某些样品可能在残留方面表现出一定的活性。从ELISA的结果来看，采用0.30ppb的临界值可以获得较低的假阳性结果，这表明该技术为合成代谢样品的检测和筛选提供了一种快速可靠的方法。对所有样品（阴性和阳性）进行GC-MS分析，以进行确证。GC-MS分析结果为阴性。这些结果表明，上述观察和报告的活性是由于样品的基质，而不是由于活性雌激素化合物。还报告了提取回收率和变异系数的数据。
A total of 146 sheep urine and 87 chicken muscle samples collected from birds sold in the State of Kuwait local market (originating from Brazil, Denmark, France and Turkey) were tested for residues of two xenobiotic estrogenic compounds, namely diethylstilbestrol (DES); and ethinylestradiol. The samples were analyzed using enzyme-linked immunosorbent assay (ELISA) and gas chromatography–mass spectrometry (GC–MS). Data obtained showed that the levels of DES and ethinylestradiol in the urine ranged from not-detected (ND) to 1.2 and ND to 0.90ppb, respectively. In the chicken muscle, the levels encountered were ND to 0.70 and ND to 0.30ppb, respectively, indicating that some samples may show some activity in terms of a residue, as recommended by international organizations. In view of the results obtained by ELISA, the employment of a cut-off value of 0.30ppb would make it reasonable to obtain low false-positive results, thus indicating that such a technique provides a fast and reliable method for the detection and screening of anabolic samples. All samples (both negative and positive) were subjected to GC–MS analysis for confirmatory purposes. The results obtained from the GC–MS analysis were found to be negative. These results show that the activity seen and reported above was due to the matrix of the samples, but not due to the active estrogenic compounds. Data on extraction recovery and coefficient of variation are also reported.