Inhibition of Helicobacter pylori and associated urease by oregano and cranberry phytochemical synergies
Inhibition of Helicobacter pylori and associated urease by oregano and cranberry phytochemical synergies
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DOI：
10.1128/aem.71.12.8558-8564.2005
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发表时间：
2005-12-01
影响因子：
4.4
通讯作者：
Shetty, K
中科院分区：
文献类型：
--
作者：
Lin, YT;Kwon, YI;Shetty, K
溃疡相关性消化不良是由幽门螺杆菌感染引起的。H.幽门与大多数消化性溃疡有关。抗生素治疗并不总是抑制或杀死H。pylori有潜在的抗生素耐药性。本研究的目的是确定使用酚类植物化学提取物抑制H。pylori在实验室培养基中。我们的方法涉及一个特定的酚类配置文件的发展与优化不同比例的提取物混合物从牛至和蔓越莓。随后，评价了抗微生物活性和抗微生物连接的脲酶抑制能力。结果表明，提取物混合物中的抗菌活性大于每个物种的单独提取物。结果还表明，牛至和蔓越莓酚类化合物的协同作用可能是更重要的抑制比任何物种特定的酚浓度。此外，基于平板试验，可能的作用模式可能是通过脲酶抑制和通过抑制质膜上的脯氨酸脱氢酶破坏能量产生。
Ulcer-associated dyspepsia is caused by infection with Helicobacter pylori. H. pylori is linked to a majority of peptic ulcers. Antibiotic treatment does not always inhibit or kill H. pylori with potential for antibiotic resistance. The objective of this study was to determine the potential for using phenolic phytochemical extracts to inhibit H. pylori in a laboratory medium. Our approach involved the development of a specific phenolic profile with optimization of different ratios of extract mixtures from oregano and cranberry. Subsequently, antimicrobial activity and antimicrobial-linked urease inhibition ability were evaluated. The results indicated that the antimicrobial activity was greater in extract mixtures than in individual extracts of each species. The results also indicate that the synergistic contribution of oregano and cranberry phenolics may be more important for inhibition than any species-specific phenolic concentration. Further, based on plate assay, the likely mode of action may be through urease inhibition and disruption of energy production by inhibition of proline dehydrogenase at the plasma membrane.