Comprehensive assessment of chemical residues in surface and wastewater using passive sampling, chemical, biological, and fish behavioral assays
Comprehensive assessment of chemical residues in surface and wastewater using passive sampling, chemical, biological, and fish behavioral assays
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使用被动采样、化学、生物和鱼类行为测定对地表和废水中的化学残留物进行综合评估
DOI：
10.1016/j.scitotenv.2022.154176
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发表时间：
2022
影响因子：
9.8
通讯作者：
Aga, Diana S.
中科院分区：
文献类型：
--
作者：
Brunelle, Laura D.;Huang, Irvin J.;Angeles, Luisa F.;Running, Logan S.;Sirotkin, Howard I.;McElroy, Anne E.;Aga, Diana S.
采用液相色谱-串联质谱法（LC/MS/MS）分析了10个城市污水处理厂（WWTPs）排放到哈德逊河、地表沃茨和野生鱼类样品中的污水，以研究污水排放对新兴关注污染物（CEC）浓度及其对鱼类生态影响的影响。分析基于41种药物的靶向检测和CEC的非靶向分析（可疑筛选）。采用斑马鱼幼鱼游泳行为试验对污水处理厂出水的生物学效应进行了评价。采用抓斗采样和极性有机化学综合采样器（POCIS）测定了地表沃茨中的残留物浓度。此外，卵黄蛋白原肽，作为内分泌干扰的生物标志物，使用LC/MS/MS定量野生捕捞鱼血浆样品。共鉴定出94种化学残留物，包括63种药物、10种工业化学品和21种农药。在100%的流出物样品中检测到8种靶向药物，中位检测结果为：安非他酮（194 ng/L）、卡马西平（91 ng/L）、环丙沙星（190 ng/L）、西酞普兰（172 ng/L）、去甲文拉法辛（667 ng/L）、碘帕醇（3790 ng/L）、扑米酮（86 ng/L）和文拉法辛（231 ng/L）。在野生捕获的鱼组织中检测到30多种化学残留物。值得注意的是，斑马鱼幼虫暴露于10个污水处理厂，在至少一个赛季，从9个污水的化学提取物，显着过度活跃。在雄性或未成熟的鱼卵黄蛋白原的表达发生2.8倍，更频繁地从哈德逊河收集的鱼相比，没有直接的污水输入的参考网站。由于在废水中检测到的药物浓度较低，很可能是测量的药物以外的化学品导致了观察到的行为变化。POCIS和非目标分析的结合使用表明，CEC检测的化学覆盖率显着增加，提供了一个更好的洞察污水处理厂的污水和农业实践对地表水水质的影响。
Effluents from ten full-scale municipal wastewater treatment plants (WWTPs) that discharge into the Hudson River, surface waters, and wild-caught fish samples were analyzed using liquid chromatography with tandem mass spectrometry (LC/MS/MS) to examine the influence of wastewater discharge on the concentrations of contaminants of emerging concern (CECs) and their ecological impacts on fish. Analysis was based on targeted detection of 41 pharmaceuticals, and non-targeted analysis (suspect screening) of CECs. Biological effects of treated WWTP effluents were assessed using a larval zebrafish (Danio rerio) swimming behavior assay. Concentrations of residues in surface waters were determined in grab samples and polar organic chemical integrative samplers (POCIS). In addition, vitellogenin peptides, used as biomarkers of endocrine disruption, were quantified using LC/MS/MS in the wild-caught fish plasma samples. Overall, 94 chemical residues were identified, including 63 pharmaceuticals, 10 industrial chemicals, and 21 pesticides. Eight targeted pharmaceuticals were detected in 100% of effluent samples with median detections of: bupropion (194 ng/L), carbamazepine (91 ng/L), ciprofloxacin (190 ng/L), citalopram (172 ng/L), desvenlafaxine (667 ng/L), iopamidol (3790 ng/L), primidone (86 ng/L), and venlafaxine (231 ng/L). Over 30 chemical residues were detected in wild-caught fish tissues. Notably, zebrafish larvae exposed to chemical extracts of effluents from 9 of 10 WWTPs, in at least one season, were significantly hyperactive. Vitellogenin expression in male or immature fish occurred 2.8 times more frequently in fish collected from the Hudson River as compared to a reference site receiving no direct effluent input. Due to the low concentrations of pharmaceuticals detected in effluents, it is likely that chemicals other than pharmaceuticals measured are responsible for the behavioral changes observed. The combined use of POCIS and non-target analysis demonstrated significant increase in the chemical coverage for CEC detection, providing a better insight on the impacts of WWTP effluents and agricultural practices on surface water quality.