A proteorhodopsin-based biohybrid light-powering pH sensor
A proteorhodopsin-based biohybrid light-powering pH sensor
复制标题
一种基于蛋白视紫红质的生物混合光动力 pH 传感器。
DOI：
10.1039/c3cp52894d
复制
发表时间：
2013-01-01
影响因子：
3.3
通讯作者：
Xiang, Yan
中科院分区：
文献类型：
--
作者：
Rao, Siyuan;Guo, Zhibin;Xiang, Yan
生物混合传感器是一种利用生物分子的本能反应或相互作用进行多功能检测的新兴技术。我们利用变形紫质（pR）的pH依赖性光电特性，开发了一种生物杂化pH传感器。瞬态吸收动力学研究表明，pR的光电行为归因于M中间体在不同环境pH值下的寿命变化。该微流控设计的pr型生物混合光动力传感器可实现pH值的实时检测，响应速度快，灵敏度高。这项工作的结果将阐明pR及其潜在的应用。
The biohybrid sensor is an emerging technique for multi-functional detection that utilizes the instinctive responses or interactions of biomolecules. We develop a biohybrid pH sensor by taking advantage of the pH-dependent photoelectric characteristics of proteorhodopsin (pR). The transient absorption kinetics study indicates that the photoelectric behavior of pR is attributed to the varying lifetime of the M intermediate at different environmental pH values. This pR-based biohybrid light-powering sensor with microfluidic design can achieve real-time pH detection with quick response and high sensitivity. The results of this work would shed light on pR and its potential applications.