Xenogenic Transplantation of Human Mesenchymal Stem Cells in a Critical Size Defect of the Sheep Tibia for Bone Regeneration
Xenogenic Transplantation of Human Mesenchymal Stem Cells in a Critical Size Defect of the Sheep Tibia for Bone Regeneration
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DOI：
10.1089/ten.tea.2009.0190
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发表时间：
2010-01-01
影响因子：
4.1
通讯作者：
Pearce, Simon
中科院分区：
文献类型：
--
作者：
Niemeyer, Philipp;Schoenberger, Thomas S.;Pearce, Simon
间充质干细胞（Mesenchymal stem cell，MSCs）是一种具有吸引力的间充质组织再生细胞。其特殊的免疫学特性表明，骨髓间充质干细胞可用于非自体应用。在这项研究中，人和绵羊MSC的再生能力进行了评估，在绵羊临界尺寸缺陷模型。将人和绵羊骨髓间充质干细胞在矿化胶原上培养，并植入3.0 cm长的绵羊胫骨骨缺损中（n = 7）。未加载的矿化胶原蛋白作为对照。通过放射学和安乐死后的组织学评估骨愈合，直至术后26周安乐死。使用人特异性原位杂交分析异种移植后人细胞的存在。放射学和组织学均显示，与未加载基质和异种治疗组相比，自体羊MSC在矿化胶原上移植后的骨形成显著更好。然而，尽管可以证明人MSC的存在，但在人MSC移植后未观察到局部或全身排斥反应。可以得出结论，尽管成功证明了异种移植后人MSC的存在，但人MSC的异种移植导致比绵羊MSC的自体移植更差的骨再生。
Mesenchymal stem cells (MSCs) represent an attractive cell population for the regeneration of mesenchymal tissues. Their special immunological characteristics suggest that MSCs could be used in nonautologous applications. In this study, the regenerative capacity of human and ovine MSCs was assessed in an ovine critical size defect model. Human and ovine MSCs from bone marrow were cultured on mineralized collagen and implanted into a 3.0-cm-long sheep tibia bone defect (n = 7). Unloaded mineralized collagen served as control. Bone healing was assessed until euthanasia 26 weeks after surgery by radiology and histologically after euthanasia. The presence of human cells after xenogenic transplantation was analyzed using human-specific in situ hybridization. Both radiology and histology demonstrated significantly better bone formation after transplantation of autologous ovine MSCs on mineralized collagen compared with unloaded matrices and with the xenogenic treatment group. Nevertheless, no local or systemic rejection reactions could be observed after transplantation of human MSCs, although the presence of human MSCs could be demonstrated. It can be concluded that despite successful demonstration of the presence of human MSCs after xenogenic transplantation, the xenogenic transplantation of human MSCs leads to poorer bone regeneration than autologous transplantation of ovine MSCs.