DEHP, bis(2)-ethylhexyl phthalate, alters gene expression in human cells: possible correlation with initiation of fetal developmental abnormalities
DEHP, bis(2)-ethylhexyl phthalate, alters gene expression in human cells: possible correlation with initiation of fetal developmental abnormalities
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DOI：
10.1177/0960327106071977
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发表时间：
2006-12-01
影响因子：
2.8
通讯作者：
Busbee, D. L.
中科院分区：
文献类型：
--
作者：
Hokanson, R.;Hanneman, W.;Busbee, D. L.
邻苯二甲酸二乙基己酯 (DEHP) 是一种分布广泛的邻苯二甲酸盐，由于其多种商业和制造用途，人类会接触到它。作为一种增塑剂，它存在于多种产品中，并且在接受邻苯二甲酸盐筛查的人的尿样中检测到了 DEHP 的代谢物。我们利用 DNA 微阵列分析来评估 DEHP 对人类细胞系 MCF-7 的基因表达干扰活性，发现 DEHP 在我们使用的 NEN2400 芯片上发现的 2400 个基因中，大约 34% 的基因显着失调。结果表明，DEHP（一种已知的雌激素激动剂和可能的雄激素拮抗剂）会改变许多基因的表达，其中许多基因对胎儿发育至关重要。
Diethylhexylphthalate (DEHP) is a widely distributed phthalate, to which humans are exposed to due to its variety of commercial and manufacturing uses. As a plasticiser, it is found in a wide number of products, and metabolites of DEHP have been detected in urine samples from a high percentage of the people screened for phthalates. We utilised DNA microarray analysis to evaluate DEHP for gene expression disrupting activity using the human cell line MCF-7, and found that DEHP significantly dysregulated approximately 34% of the 2400 genes spotted on the NEN2400 chip we used. The results suggest that DEHP, a known estrogen agonist and probable androgen antagonist, alters the expression of a number of genes, many of which are critical for fetal development.