Use of circumsporozoite protein enzyme-linked immunosorbent assay compared with microscopic examination of salivary glands for calculation of malaria infectivity rates in mosquitoes (Diptera: culicidae) from Cameroon
Use of circumsporozoite protein enzyme-linked immunosorbent assay compared with microscopic examination of salivary glands for calculation of malaria infectivity rates in mosquitoes (Diptera: culicidae) from Cameroon
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DOI：
10.1603/0022-2585-38.3.451
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发表时间：
2001-05-01
影响因子：
2.1
通讯作者：
Tchuinkam, T
中科院分区：
文献类型：
--
作者：
Fontenille, D;Meunier, JY;Tchuinkam, T
喀麦隆的一项调查比较了环孢子虫蛋白酶联免疫吸附试验（CSP ELISA）与按蚊唾液腺解剖和显微镜检查在测量按蚊传染性方面的实用性。对4种375只雌虫的唾液腺进行了孢子虫检查。镜检腺体为M；并对所有剩余的头部和胸部进行ELISA检测。ELISA检测的灵敏度为100%(18/18)，置信区间（CI）为78.1 ~ 100，特异性为99.7%（357/358）。Cl(98.2 -100)。Kappa值与唾液腺ELISA检测结果一致，为0.97。头胸CSP ELISA法高估了唾液腺真实感染率为12.0%。在非洲中部一个多年生传播的村庄所获得的结果，证明了采用ELISA法测定昆虫接种率是合理的。
A survey in Cameroon compared the usefulness of the circumsporozoite protein enzyme-linked immunosorbent assay (CSP ELISA) to dissection and microscopic examination of anopheline salivary glands for measuring infectivity rates in anopheline mosquitoes. The salivary glands of 375 females, belonging to four species were examined for sporozoites. After microscopic examination, the glands as M;ell as all the remaining heads and thoraces were tested by ELISA. The sensitivity of ELISA was 100% (18/18), confidence interval (CI) (78.1-100) and the specificity was 99.7% (357/358). Cl (98.2-100). The Kappa value, agreement between examination of the glands and salivary gland ELISA, was 0.97. The head-thorax CSP ELISA overestimated the true salivary gland infection rate ty 12.0%. The results obtained in Central Africa in a village with perennial transmission highly, justified the use of the ELISA for measuring the entomological inoculation rate.