The proatherogenic cytokine interleukin-18 is secreted by human adipocytes
The proatherogenic cytokine interleukin-18 is secreted by human adipocytes
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DOI：
10.1530/eje.1.01897
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发表时间：
2005-06-01
影响因子：
5.8
通讯作者：
Herder, C
中科院分区：
文献类型：
--
作者：
Skurk, T;Kolb, H;Herder, C
目的：白细胞介素（IL）-18是先天免疫的重要介质，也是心血管疾病发展的重要危险因素，最近发现肥胖患者IL-18水平升高。本研究的目的是研究人脂肪细胞是否产生IL-18。方法：人脂肪组织取自接受择期整形手术的瘦女性和接受腹腔镜手术（胃束带术）的肥胖个体。从乳腺脂肪组织中分离前脂肪细胞，并在规定的成脂条件下分化。通过实时逆转录酶PCR分析IL-18表达。结果：所有分化阶段的人前脂肪细胞均自发分泌IL-18。同时检测到显著量的IL-18 mRNA。新鲜分离的成熟脂肪细胞从皮下和网膜仓库也释放IL-18。IL-18释放从脂肪细胞从肥胖的捐助者是约3倍高的脂肪细胞相比，从非肥胖的donators.Conclusions：我们的结论是，人体脂肪组织产生IL-18，从而有助于全身IL-18浓度。这一发现支持了脂肪细胞表现为原始免疫细胞的概念，并且IL-18可能介导肥胖的一些有害并发症，如心血管疾病和2型糖尿病。欧洲内分泌学杂志。
Objective: Interleukin (IL)-18, an important mediator of innate immunity and strong risk factor for the development of cardiovascular disease, was shown recently to be elevated in obesity. The aim of our study was to investigate whether human adipocytes produce IL-18.Methods: Human adipose tissue was obtained from lean women undergoing elective plastic surgery and from obese individuals undergoing laparoscopic surgery (gastric,banding). Preadipocytes from mammary adipose tissue were isolated and differentiated under defined adipogenic conditions. IL-18 expression was analyzed by real-time reverse transcriptase PCR. ELISA and immunocytochemistry.Results: Human preadipocytes of all differentiation stages spontaneously secreted IL-18. In parallel significant amounts of IL-18 mRNA were detected. Freshly isolated mature adipocytes from subcutaneous and omental depots also released IL-18. IL-18 release from adipocytes from obese donors was about 3-fold higher compared to adipocytes from non-obese donors.Conclusions: We conclude that human adipose tissue produces IL-18 and thereby contributes to systemic IL-18 concentrations. This finding supports the concepts that adipocytes behave as primitive immune cells and that IL-18 may mediate some of the detrimental complications of obesity such as cardiovascular disease and type 2 diabetes. European Journal of Endocrinology.