Analysis of bacterial and fungal communities in continuous- cropping ramie (Boehmeria nivea L.Gaud) fields in different areas in China
Analysis of bacterial and fungal communities in continuous- cropping ramie (Boehmeria nivea L.Gaud) fields in different areas in China
复制标题
我国不同地区连作苎麻田细菌和真菌群落分析
DOI：
10.1038/s41598-020-58608-0
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Zhusiyuan
中科院分区：
文献类型：
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作者：
Wangyanzhou;Xu xiaomin;Liutouming;Wanghong wu;Yangyan;Chenxiaorong;Zhusiyuan
苎麻(Boehmeria niveaL.)Gaud)长期连作。在这里，我们利用Illumina高通量测序技术，对元江、咸宁、四川和江西等地的麻田连作相关细菌和真菌进行鉴定。稀释度结果表明，江西的细菌α多样性明显低于其他地区。真菌门中优势菌门为子囊菌门、担子菌门和接合菌门。在江西，微生物占所有有效读数的79.03%，这可能会显著降低微生物多样性，并对连作产生负面影响。我们使用传统的方法来检测土壤养分。四川土壤pH和速效P、K含量较高，全N含量较低；江西土壤pH和速效P、K含量较低。冗余度分析表明，脲酶活性、PH值、速效K和全N与细菌群落丰度显著相关，而全N与真菌群落丰度显著相关(P <； 0.0 1)。总体而言，土壤环境因子对连作苎麻细菌多样性的影响大于对真菌多样性的影响。今后将重点研究根际细菌的作用，解决苎麻连作障碍。
Ramie (Boehmeria niveaL. Gaud) suffers from long-term continuous cropping. Here, using Illumina high-throughput sequencing technology, we aimed to identify bacteria and fungi associated with continuous cropping in ramie fields in Yuanjiang, Xianning, Sichuan, and Jiangxi. The rarefaction results showed that Jiangxi had significantly lower bacterial α-diversity than that of the other areas. Firmicutes, Proteobacteria, and Acidobacteria were the dominant bacterial phyla, and Ascomycota, Basidiomycota, and Zygomycota were the dominant fungal phyla. In Jiangxi, Firmicutes accounted for 79.03% of all valid reads, which could have significant decreased microbial diversity and negative effects of continuous ramie cropping. We used traditional methods to examine soil nutrients. Sichuan had a relatively high pH and available P and K, but low total N; opposite findings were recorded in Jiangxi. The redundancy analysis revealed that the urease activity, PH, available K, and total N significantly correlated with bacterial community abundance, whereas only total N significantly correlated with fungal community abundance (P < 0.01). Overall, the effect of soil environmental factors on the bacterial diversity of continuous ramie cropping was greater than that on fungal diversity. In the future, we will focus on the effect of rhizosphere bacteria to solve the obstacle in continuous ramie cropping.