Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle
Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle
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DOI：
10.1007/s00335-007-9072-y
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发表时间：
2007-12-01
期刊：
影响因子：
2.5
通讯作者：
Mannen, Hideyuki
中科院分区：
文献类型：
--
作者：
Hoashi, Shogo;Ashida, Nobuhisa;Mannen, Hideyuki
为了研究影响牛肉胴体脂肪酸组成的遗传因素，我们研究了牛脂肪组织中硬脂酰辅酶A去饱和酶（SCD）的遗传特征及其对脂肪酸组成的影响。已知固醇调节元件结合蛋白（sterol regulatory element binding protein，SREBP）是调节SCD和与组织中脂质和脂肪酸代谢相关的其它基因的基因表达水平的转录因子。因此，我们确定了牛SREBP-1 cDNA的全长序列，然后调查了牛基因组中整个外显子和内含子的多态性。在日本黑牛SREBP-1基因内含子5中发现了84 bp的大片段插入（长型：L）和缺失（短型：S），但在外显子区域没有发现明显的突变。利用606头日本黑牛的基因组DNA和胴体性状信息，分析了SREBP-1基因型与脂肪酸组成/脂肪熔点之间的关联。S型导致肌内脂肪中单不饱和脂肪酸（MUFA）比例高1.3%，熔点低1.6 ℃。牛SREBP-1的基因分型被认为反映了与日本黑牛脂肪组织生理特性相关的遗传变异。
To investigate genetic factors that affect fatty acid composition in beef carcass, we previously investigated genetic profiles of stearoyl-CoA desaturase (SCD) and their effect on fatty acid composition in fat tissue of cattle. It has been known that sterol regulatory element binding protein (SREBP) is a transcription factor that regulates gene expression levels of SCD and other genes relevant to lipid and fatty acid metabolism in tissue. Therefore, we determined the full-length sequence of bovine SREBP-1 cDNA and then surveyed polymorphisms in whole exons and introns in the bovine genome. Large 84-bp insertion (long type: L) and deletion (short type: S) were found in intron 5 of bovine SREBP-1 in Japanese Black cattle, although there was no notable mutation in exon regions. The associations between the SREBP-1 genotypes and fatty acid compositions/fat melting points were analyzed by using genomic DNA with carcass trait information from 606 Japanese Black cattle. The S type contributed to 1.3% higher monounsaturated fatty acid (MUFA) proportion and 1.6 degrees C lower melting point in intramuscular fat. Genotyping of bovine SREBP-1 is considered to reflect a genetic variation which is associated with physiologic characteristics of fat tissue in Japanese black cattle.