Characterization and culture of sinusoidal endothelium from normal rat liver: lipoprotein uptake and collagen phenotype.
Characterization and culture of sinusoidal endothelium from normal rat liver: lipoprotein uptake and collagen phenotype.
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DOI：
10.1016/0016-5085(84)90188-4
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发表时间：
1984-12
期刊：
影响因子：
29.4
通讯作者：
M. G. Irving;F. Roll;Sandra Huang;D. Bissell
中科院分区：
文献类型：
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作者：
M. G. Irving;F. Roll;Sandra Huang;D. Bissell
通过离心淘洗从成年大鼠肝脏中分离出正弦内皮细胞，并在原代培养物中建立。他们的鉴定利用了这些细胞的一种新过程，即人类乙酰乙酰化低密度脂蛋白的隔离。体内给药后，肝脏内标记的乙酰乙酰化低密度脂蛋白仅与肝窦内皮细胞相关，并且当用稳定的荧光化合物 3,3'-二十八烷基碳花青标记时，提供了一种通过荧光显微镜识别分离细胞的方法。在内皮细胞培养物中，90% 的细胞发出荧光并呈现窗孔。通过免疫荧光方法评估培养物的胶原表型，仅显示细胞相关的 IV 型胶原； I、III 和 V 型无法检测到。在其他研究中，发现这些细胞缺乏因子 VIII-R 抗原和 Weibel-Palade 小体，这进一步证明它们与大血管和其他毛细血管内皮细胞有很大不同。
Sinusoidal endothelial cells from adult rat liver have been isolated by centrifugal elutriation and been established in primary culture. Their identification has made use of a novel process of these cells, the sequestration of human acetoacetylated lowdensity lipoprotein. After its administration in vivo, labeled acetoacetylated low-density lipoprotein within the liver was associated solely with sinusoidal endothelial cells and, when labeled with the stable fluorescent compound 3,3′-dioctadecylindocarbocyanine, provided a means of identifying isolated cells by fluorescence microscopy. In endothelial cell cultures, 90% of the cells were fluorescent and exhibited fenestrae. The collagen phenotype of cultures was assessed by an immunofluorescent approach, which revealed cell-associated type IV collagen only; types I, III, and V were undetectable. In other studies, it was found that these cells lacked factor VIII-R antigen and Weibel-Palade bodies, adding to the evidence that they differ substantially from large-vessel and other capillary endothelia.