Chromatin structure and de novo methylation of sperm DNA: implications for activation of the paternal genome.
Chromatin structure and de novo methylation of sperm DNA: implications for activation of the paternal genome.
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精子 DNA 的染色质结构和从头甲基化：对父本基因组激活的影响。
DOI：
10.1126/science.2986289
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Conkin,KF
中科院分区：
文献类型：
--
作者：
Groudine,M;Conkin,KF
组成型表达基因、组织特异性基因和失活基因的染色质结构特征在鸡精子染色质中不存在。然而，组成型表达基因中精子DNA甲基化不足的位点，而不是珠蛋白基因或非活性基因中的位点，对应于体细胞组织和精原细胞中染色质结构改变的区域（超敏位点）。在精原细胞阶段和第一次减数分裂前期之间发生了一个从头甲基化过程，其中这些基因内和侧翼的区域被甲基化，但不包括超敏感位点内序列的甲基化。这些甲基化不足的区域可能在胚胎发生过程中激活父本基因组中发挥作用。
The chromatin structure characteristic of constitutively expressed genes, tissue-specific genes, and inactive genes is absent in chicken sperm chromatin. However, point sites of undermethylation in sperm DNA within constitutively expressed genes, but not within globin genes or an inactive gene, correspond to the location of regions of altered chromatin structure (hypersensitive sites) in somatic tissue and spermatogonial cells. A de novo methylation process whereby regions within and flanking these genes become methylated, but which excludes the methylation of sequences within hypersensitive sites, occurs between the spermatogonial stage and the first meiotic prophase. These undermethylated regions may play a role in the activation of the paternal genome during embryogenesis.