Fabrication of nanoelectrode ensembles by electrodepositon of Au nanoparticles on single-layer graphene oxide sheets.
Fabrication of nanoelectrode ensembles by electrodepositon of Au nanoparticles on single-layer graphene oxide sheets.
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DOI：
10.1039/c2nr30142c
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发表时间：
2012-03
期刊：
影响因子：
6.7
通讯作者：
Zhijuan Wang;Juan Zhang;Z. Yin;Shixin Wu;D. Mandler;Hua Zhang
中科院分区：
文献类型：
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作者：
Zhijuan Wang;Juan Zhang;Z. Yin;Shixin Wu;D. Mandler;Hua Zhang
通过在单层氧化石墨烯(GO)片上电沉积金纳米颗粒(AuNPs)，在玻璃碳电极(GCE)上制备了纳米电极团簇(Nee)。由于AuNPs在GCE上的覆盖密度低，且它们之间的距离很大，所以所制备的NeES呈现出典型的S形伏安曲线，这可以通过改变电沉积时间来轻松控制。作为概念验证，将探针HS-DNA通过Au-S键固定在NeES上后，用亚甲基蓝嵌入器检测目标DNA。结果表明，在5μ的L溶液中，可检测到低至100fM，即0.5amol的DNA。
Nanoelectrode ensembles (NEEs) have been fabricated by the electrodeposition of Au nanoparticles (AuNPs) on single-layer graphene oxide (GO) sheets coated on a glassy carbon electrode (GCE). The fabricated NEEs show a typical sigmoidal shaped voltammetric profile, arising from the low coverage density of AuNPs on GCE and large distance among them, which can be easily controlled by varying the electrodeposition time. As a proof of concept, after the probe HS-DNA is immobilized on the NEEs through the Au-S bonding, the target DNA is detected with the methylene blue intercalator. Our results show that the target DNA can be detected as low as 100 fM, i.e. 0.5 amol DNA in 5 μL solution.