Analysis of Drosophila melanogaster Lifespan.
Analysis of Drosophila melanogaster Lifespan.
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DOI：
10.1007/978-1-0716-0592-9_4
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Tower J
中科院分区：
文献类型：
--
作者：
Landis GN;Doherty D;Tower J
实验室果蝇Drosophila melanogaster是研究衰老的主要模型之一。尽管已经确定了果蝇衰老的几种行为和生理生物标志物，但寿命仍然是衰老率的最可靠指标。衰老和寿命可以通过遗传改变以及药物和饮食成分来调节，以揭示衰老的基本和保守机制。本文介绍了果蝇寿命测定的方法，包括培养基的制备、培养基中添加不同药物、果蝇的培养、果蝇的传代和死亡记录以及寿命数据的分析。
The laboratory fruit fly Drosophila melanogaster is one of the leading models for the study of aging. Whereas several behavioral and physiological biomarkers of aging have been identified for Drosophila, life span remains the most robust measure of aging rate. Aging and life span can be modulated by genetic alterations, as well as by drugs and dietary components, to reveal basic and conserved mechanisms of aging. Here methods are presented for Drosophila life span assay, including media preparation, supplementation of media with various drugs, culturing of the flies, passaging flies and recording deaths, and the analysis of life span data.