Enzyme-gold nanoparticle bioconjugates: quantification of particle stoichiometry and enzyme specific activity.
Enzyme-gold nanoparticle bioconjugates: quantification of particle stoichiometry and enzyme specific activity.
复制标题
酶-金纳米颗粒生物结合物：颗粒化学计量和酶比活性的量化。
DOI：
10.1007/978-1-62703-468-5_13
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Keating,ChristineD
中科院分区：
文献类型：
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作者：
Keighron,JacquelineD;Keating,ChristineD
酶-金纳米颗粒生物缀合物具有从纳米反应器到传感器和模型系统的各种各样的用途。虽然容易制造，但这些生物缀合物通常没有很好地表征。该方案概述了酶-纳米颗粒生物缀合物的制备和用于定量酶的两种互补方法：纳米颗粒化学计量，从该化学计量可以确定吸附的酶的比活性。诸如此类的表征可以帮助研究人员改进未来生物缀合物系统的设计和应用。
Enzyme–gold nanoparticle bioconjugates have a wide variety of uses ranging from nanoreactors to sensors and model systems. While easy to make, these bioconjugates are often not well characterized. This protocol outlines preparation of enzyme–nanoparticle bioconjugates and two complementary methods for quantifying enzyme:nanoparticle stoichiometry from which the specific activity of the adsorbed enzymes can be determined. Characterizations such as these can aid researchers in improving the design and application of future bioconjugate systems.