A new effective process for production of curdlan oligosaccharides based on alkali-neutralization treatment and acid hydrolysis of curdlan particles in water suspension
A new effective process for production of curdlan oligosaccharides based on alkali-neutralization treatment and acid hydrolysis of curdlan particles in water suspension
复制标题
基于水悬浮液中凝胶多糖颗粒碱中和酸解处理生产凝胶多糖低聚糖的有效新工艺
DOI：
10.1007/s00253-013-5125-1
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发表时间：
2013-10-01
影响因子：
5
通讯作者：
Li, Wei-Jiang
中科院分区：
文献类型：
--
作者：
Li, Jing;Zhu, Li;Li, Wei-Jiang
具有生物活性的β-1，3-寡糖是由可得兰多糖水解而成，其生物医学应用迅速增长。不溶于水的凝胶多糖阻碍了其水解效率，我们新开发的碱中和处理工艺提高了其水解效率，使凝胶多糖悬浮液的稳定性提高到20天以上，而未处理的对照在5分钟内沉降。基于沉降和扫描电镜分析，提出了可得然胶颗粒的层结构模型，该模型由致密的核心和水合的外层组成显微镜观察该模型得到了验证，通过单一和两步酸水解，指示水解的敏感性降低时，接近致密的核心。电喷雾离子化质谱，薄层色谱分析，和有效的HPLC程序导致改进的工艺的发展，以生产纯化的个别β-1，3-寡糖的聚合度从2到10和潜在的生物医学应用从可得兰水解产物。我们新的可得兰低聚糖生产工艺提供了比以前公布的工艺更好的替代品。
Biologically active beta-1,3-oligosaccharides with rapidly growing biomedical applications are produced from hydrolysis of curdlan polysaccharide. The water-insoluble curdlan impedes its hydrolysis efficiency which is enhanced by our newly developed alkali-neutralization treatment process to increase the stability of curdlan suspension to more than 20 days, while the untreated control settled within 5 min. A putative double-layer structure model comprising of a compact core and a hydrated outer layer was proposed to describe the treated curdlan particles based on sedimentation and scanning electron microscopy observation. This model was verified by single- and two-step acid hydrolysis, indicative of the reduced susceptibility to hydrolysis when close to the compact core. Electrospray ionization-mass spectrometry, thin-layer chromatography analyses, and effective HPLC procedure led to the development of improved process to produce purified individual beta-1,3-oligosaccharides with degrees of polymerization from 2 to 10 and potential for biomedical applications from curdlan hydrolyzate. Our new curdlan oligosaccharide production process offers an even better alternative to the previously published processes.