Molecular analysis of cellulose biosynthesis in Arabidopsis
Molecular analysis of cellulose biosynthesis in Arabidopsis
复制标题
DOI：
10.1126/science.279.5351.717
复制
发表时间：
1998-01-30
期刊：
影响因子：
56.9
通讯作者：
Williamson, RE
中科院分区：
文献类型：
--
作者：
Arioli, T;Peng, LC;Williamson, RE
纤维素是一种丰富的结晶多糖，是植物形态发生和许多工业的核心。化学和超微结构分析以及图位克隆表明，拟南芥RSW 1基因座编码纤维素合成酶的催化亚基。克隆的基因补充了rsw 1突变体，该突变体的温度敏感等位基因在一个氨基酸中发生了变化。突变等位基因导致纤维素合成的特异性减少、非结晶β-1，4-葡聚糖的积累、纤维素合酶的分解和广泛的形态异常。微纤维结晶可能需要RSW 1基因产物适当组装成合酶复合物，而葡聚糖生物合成本身不需要。
Cellulose, an abundant, crystalline polysaccharide, is central to plant morphogenesis and to many industries. Chemical and ultrastructural analyses together with map-based cloning indicate that the RSW1 locus of Arabidopsis encodes the catalytic subunit of cellulose synthase. The cloned gene complements the rsw1 mutant whose temperature-sensitive allele is changed in one amino acid. The mutant allele causes a specific reduction in cellulose synthesis, accumulation of noncrystalline beta-1,4-glucan, disassembly of cellulose synthase, and widespread morphological abnormalities. Microfibril crystallization may require proper assembly of the RSW1 gene product into synthase complexes whereas glucan biosynthesis per se does not.