Topographically specific regeneration of sensory axons in the spinal cord
Topographically specific regeneration of sensory axons in the spinal cord
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DOI：
10.1073/pnas.1003287107
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发表时间：
2010-06-22
影响因子：
11.1
通讯作者：
Frank, Eric
中科院分区：
文献类型：
--
作者：
Harvey, Pamela;Gong, BangJian;Frank, Eric
Artemin是胶质源性神经营养因子家族的一员，可促进背根损伤后感觉轴突的再生。我们在这里报告说，几类感觉轴突再生到地形适当的区域的背角与artemin治疗。再生肌肉和皮肤有髓感觉传入的投射仅限于正确的脊髓节段和脊髓灰质内的适当区域。再生的无髓轴突表达降钙素基因相关肽项目只到浅层的背角，未受伤的伤害性传入项目正常。相比之下，脑室内输注Nogo受体的可溶性形式，阻断几种髓鞘相关抑制蛋白的作用，促进整个脊髓背侧白色和灰质的感觉轴突相对不受限制的再生。这些结果表明，线索能够引导再生轴突适当的脊髓目标持续在成年哺乳动物的脊髓，但只有一些刺激再生的方法允许使用这些线索生长轴突。
Artemin, a member of the glial-derived neurotrophic factor family, promotes robust regeneration of sensory axons after dorsal root crush. We report here that several classes of sensory axons regenerate to topographically appropriate regions of the dorsal horn with artemin treatment. Projections of regeneratedmuscle and cutaneous myelinated sensory afferents are restricted to the correct spinal segments and to appropriate regions within spinal gray matter. Regenerated unmyelinated axons expressing calcitonin gene-related peptide project only to superficial laminae of the dorsal horn, where uninjured nociceptive afferents project normally. In contrast, intraventricular infusion of a soluble form of the Nogo receptor that blocks the action of several myelin-associated inhibitory proteins promotes relatively unrestricted regeneration of sensory axons throughout the dorsal white and gray matter of the spinal cord. These results demonstrate that cues capable of guiding regenerating axons to appropriate spinal targets persist in the adult mammalian cord, but only some methods of stimulating regeneration allow the use of these cues by growing axons.