HTLV-III in cells cultured from semen of two patients with AIDS.
HTLV-III in cells cultured from semen of two patients with AIDS.
复制标题
两名艾滋病患者精液培养细胞中的 HTLV-III。
作者：
D. Zagury;J. Bernard;J. Leibowitch;B. Safai;J. Groopman;M. Feldman;M. Sarngadharan;R. Gallo
流行病学结果表明，获得性免疫缺陷综合征（艾滋病）的病原体主要通过血液制品、精液和唾液传播。有证据表明，人类T细胞白血病（嗜淋巴细胞）病毒III型（HTLV-III）是这种药物。HTLV-III已经从T细胞中反复分离出来，T细胞是从AIDS和AIDS前期患者以及据信已经暴露于病毒的健康人的外周血或淋巴结组织中获得的。在本研究中，HTLV-III被检测到，并从艾滋病患者的精液中存在的T细胞分离。在T细胞生长因子（白细胞介素-2）的存在下培养来自AIDS患者和正常个体的精液的单核细胞。6 ~ 8天后，来自AIDS患者的细胞瞬时表达HTLV-III抗原，而来自正常个体的细胞则不表达。当艾滋病患者精液中的单核细胞与允许的人T细胞系共培养时，产生的细胞培养物表达高水平的逆转录酶活性，通过电子显微镜观察显示逆转录病毒颗粒，并且当使用针对HTLV-III的p24和p15抗原的单克隆抗体通过固定细胞间接免疫荧光检测时，HTLV-III特异性抗原呈阳性。
Epidemiological results suggest that the etiological agent of the acquired immune deficiency syndrome (AIDS) is transmitted primarily through blood products, semen, and saliva. There is evidence that the human T-cell leukemia (lymphotropic) virus type III (HTLV-III) is this agent. HTLV-III has been isolated repeatedly from T cells obtained from peripheral blood or lymph node tissue of AIDS and pre-AIDS patients and of healthy people believed to have been exposed to the virus. In the present study, HTLV-III was detected in and isolated from T cells present in the seminal fluid of AIDS patients. Mononuclear cells from the semen of AIDS patients and normal individuals were cultured in the presence of T-cell growth factor (interleukin-2). After 6 to 8 days, HTLV-III antigens were transiently expressed by the cells from the AIDS patients but not by those from the normal individuals. When the mononuclear cells from the semen of AIDS patients were cocultured with a permissive human T-cell line, cell cultures were produced that expressed high levels of reverse transcriptase activity, showed retroviral particles by electron microscopy, and were positive for HTLV-III-specific antigens when tested by fixed-cell indirect immunofluorescence with the use of monoclonal antibodies to the p24 and p15 antigens of HTLV-III.