MECHANISM OF WALL SYNTHESIS IN BACTERIA - ORGANIZATION OF ENZYMES AND ISOPRENOID PHOSPHATES IN MEMBRANE
MECHANISM OF WALL SYNTHESIS IN BACTERIA - ORGANIZATION OF ENZYMES AND ISOPRENOID PHOSPHATES IN MEMBRANE
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DOI：
10.1042/bj1270011
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发表时间：
1972-01-01
影响因子：
4.1
通讯作者：
HUSSEY, H
中科院分区：
文献类型：
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作者：
ANDERSON, RG;BADDILEY, J;HUSSEY, H
1.地衣芽孢杆菌无细胞制剂合成肽聚糖和磷壁酸9945和枯草芽孢杆菌N.C.T.C. 3610已经在各种条件下进行了研究。2.结果表明，聚（甘油磷酸酯）是通过脂质中间体合成的，并且从这项工作和其他工作中得出结论，所有主要的细菌壁聚合物都是通过这种中间体以类似的方式形成的。3.证明了肽聚糖和磷壁酸的合成之间的密切相互关系，抑制研究证实，参与肽聚糖合成的聚戊烯醇磷酸分子与合成磷壁酸的系统共享。4.用于合成一种聚合物的核苷酸抑制另一种聚合物的合成，并且这些作用可以通过将酶系统与适当的核苷酸前体预孵育来增强或减弱。5.它的结论是，返回的磷酸十一异戊烯醇到一个共同的游泳池只发生在聚合物链完成后，而不是在每个周期后，在个别重复单元的附件。这个和其他观察结果支持了细菌壁合成的模型，其中每个聚合物的多酶系统在膜中紧密对齐，其中磷酸十一烯醇分子以使其能够共享的方式位于它们之间。讨论了壁合成的一般机理及其控制。
1. The synthesis of peptidoglycan and teichoic acids by cell-free preparations fromBacillus licheniformisA.T.C.C. 9945 andBacillus subtilisN.C.T.C. 3610 has been studied under a variety of conditions. 2. It was shown that poly(glycerol phosphate) is synthesized through a lipid intermediate, and it is concluded from this and other work that all major bacterial wall polymers are formed in a similar manner through such intermediates. 3. Close interrelation between the synthesis of peptidoglycan and teichoic acids was demonstrated, and inhibition studies confirm that the polyprenol phosphate molecules participating in the synthesis of peptidoglycan are shared with the systems that synthesize teichoic acids. 4. Nucleotides for the synthesis of one polymer are inhibitory towards synthesis of the other, and these effects can be enhanced or diminished by preincubation of the enzyme system with appropriate nucleotide precursors. 5. It is concluded that the return of undecaprenol phosphate to a common pool occurs only after the completion of polymer chains, and not after each cycle in the attachment of individual repeating units. This and other observations support a model for bacterial wall synthesis in which the multi-enzyme systems for each polymer are closely aligned in the membrane, with a molecule of undecaprenol phosphate located between them in a manner that enables it to be shared. The general mechanisms of wall synthesis and its control are discussed.