Measurement of Recent Exposure to Phlebotomus argentipes, the Vector of Indian Visceral Leishmaniasis, by Using Human Antibody Responses to Sand Fly Saliva
Measurement of Recent Exposure to Phlebotomus argentipes, the Vector of Indian Visceral Leishmaniasis, by Using Human Antibody Responses to Sand Fly Saliva
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DOI：
10.4269/ajtmh.2010.09-0336
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发表时间：
2010-05-01
影响因子：
3.3
通讯作者：
Rogers, Matthew E.
中科院分区：
文献类型：
--
作者：
Clements, Meredith F.;Gidwani, Kamlesh;Rogers, Matthew E.
Antibody (IgG) responses to the saliva of Phlebotomus argentipes were investigated using serum samples from regions of India endemic and non-endemic for visceral leishmaniasis (VL). By pre-adsorbing the sera against the saliva of the competing human-biting but non-VL vector P.papatasi. we significantly improved the specificity of a P.argentipes saliva enzyme-linked immunosorbent assay. Using this method, we observed a statistically significant correlation between antibodies to P argenitpes saliva and the average indoor density of female sand flies. Additionally, the method was able to detect recent changes in vector exposure when sera from VL patients were assayed before, during, and after hospitalization and protected from sand fly bites under untreated bed nets. Collectively, these results highlight the utility of antibodies to P.argentipes saliva as an important tool to evaluate VL vector control programs.
