Thyroid hormone receptor expression in the human hypothalamus and anterior pituitary
Thyroid hormone receptor expression in the human hypothalamus and anterior pituitary
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DOI：
10.1210/jc.2004-0474
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发表时间：
2005-02-01
影响因子：
5.8
通讯作者：
Fliers, E
中科院分区：
文献类型：
--
作者：
Alkemade, A;Vuijst, CL;Fliers, E
在本研究中，我们首次用免疫细胞化学方法描述了甲状腺激素受体（TR）异构体在人死后下丘脑和垂体前叶中的分布。我们使用了一套针对人类TR特异性亚型的多克隆抗血清，在6个下丘脑和垂体的连续切片上研究了TR α 1、α 2、β 1和β 2的分布。染色强度表现出强烈的个体间差异，但在基底核、室旁核和视上核中一致存在。垂体前叶可见较强的TR免疫反应性。在另外三个下丘脑中研究的基底核中神经肽Y和原黑素皮质素mrna阳性细胞似乎不表达Tits，因此，在人类中基底下丘脑中表达TRs的神经元仍有待研究。
In the present study, we describe for the first time the distribution of thyroid hormone receptor (TR) isoforms in the human postmortem hypothalamus and anterior pituitary using immunocytochemistry. We used a set of polyclonal antisera raised against the specific isoforms of the human TR. The distribution of TR alpha1, alpha2, beta1, and beta2 was studied in consecutive sections of six hypothalami and pituitaries. Staining intensity showed strong interindividual variation but was consistently present in the infundibular nucleus, paraventricular nucleus, and supraoptic nucleus. In addition, strong TR immunoreactivity was observed in the anterior pituitary. Neuropeptide Y and proopiomelanocortin mRNA-positive cells in the infundibular nucleus, which were studied in three other hypothalami, appeared not to express Tits, and thus, the neurons expressing TRs in the human mediobasal hypothalamus remain to be characterized.