ONGOING PROLIFERATION OF MELANOCYTES IN THE STRIA VASCULARIS OF ADULT GUINEA-PIGS
ONGOING PROLIFERATION OF MELANOCYTES IN THE STRIA VASCULARIS OF ADULT GUINEA-PIGS
复制标题
成年豚鼠血管纹中黑素细胞的持续增殖
DOI：
10.1016/0378-5955(94)90133-3
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发表时间：
1994-09-01
期刊：
影响因子：
2.8
通讯作者：
BENNETT, ML
中科院分区：
文献类型：
--
作者：
CONLEE, JW;GERITY, LC;BENNETT, ML
血管纹的中间细胞是来自神经嵴的黑素细胞。这些内化的色素细胞被认为是一个静态的群体，与皮肤中发现的那些不同。然而，这项调查表明，黑素细胞的成年血管纹进行连续复制。用[H-3]-胸苷放射自显影和溴脱氧尿苷（BrdU）免疫组化研究细胞增殖。在6小时内单次或多次注射[H-3]-胸苷标记平均9个中间细胞。在有色豚鼠中，每天一次注射[H-3]-胸苷2或4天，分别标记了平均24和69个中间细胞;每天一次给予BrdU 2或4天的有色豚鼠标记了平均38和75个中间细胞。相比之下，同样给予BrdU的白化病同窝仔在相同的2天和4天实验中平均仅23和42个标记的中间细胞。每24小时每mm纹的增殖细胞的平均数为1.54在有色动物和0.88在白化病。白化病豚鼠的标记中间细胞总数和密度均显著小于有色豚鼠。这些结果表明，血管纹中的黑素细胞经历连续的基线有丝分裂，并且其速率与皮肤的黑素细胞相当。这种令人惊讶的相似性促使人们推测，皮肤黑素细胞的增殖率可能受到一些已知影响皮肤中复制和色素产生的相同因素的影响。
The intermediate cells of the stria vascularis are melanocytes derived from the neural crest. These internalized pigment cells have been thought to be a static population, distinct from those found in the skin. However, this investigation demonstrates that the melanocytes of the adult stria vascularis undergo continuous replication. Cell proliferation was studied using [H-3]-thymidine autoradiography and bromodeoxyuridine (BrdU) immunohistochemistry. Single or multiple injections of [H-3]-thymidine within a six hour period labeled a mean of 9 intermediate cells. In pigmented guinea pigs, single daily injections of [H-3]-thymidine for 2 or 4 days labeled a mean of 24 and 69 intermediate cells, respectively; Pigmented guinea pigs given BrdU once daily for 2 or 4 days labeled a mean of 38 and 75 intermediate cells. By contrast, albino littermates also given BrdU averaged only 23 and 42 labeled intermediate cells in the same 2 and 4 day experiments. The mean number of proliferating cells/mm of stria per 24 h was 1.54 in the pigmented animals and 0.88 in the albinos. Both the total number and density of labeled intermediate cells were significantly smaller in the albino than the pigmented guinea pigs. These results demonstrate that the melanocytes in the stria vascularis undergo continuous baseline mitosis, and at a rate comparable to the melanocytes of the skin. This surprising similarity promotes the speculation that the proliferative rate of the strial melanocytes may be influenced by some of the same factors known to affect replication and pigment production in the skin.