CHEMILUMINESCENT FLOW METHOD FOR DETERMINATION OF FORMALDEHYDE
CHEMILUMINESCENT FLOW METHOD FOR DETERMINATION OF FORMALDEHYDE
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DOI：
10.1021/ac60363a040
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发表时间：
1975-01-01
影响因子：
7.4
通讯作者：
SLAWINSKI, J
中科院分区：
文献类型：
--
作者：
SLAWINSKA, D;SLAWINSKI, J
用碱性过氧化氢溶液氧化甲醛和没食子酸，在560-850 nm光谱范围内产生较强的化学发光(Trautz-Scholiln反应)。测定了该体系的动力学，测定了中间体和产物的化学发光光谱、吸收光谱和荧光光谱。还测定了试剂加入顺序和氧分压变化对化学发光的影响。测量了产热和溶解氧浓度随时间和试剂添加顺序的变化。从反应机理的角度对结果进行了讨论，特别强调了单重态分子氧的可能作用。测定了试剂浓度、pH、温度、流速和干扰物对最大化学发光强度的影响。化学发光强度与甲醛浓度在10~(-7)~10~(-2)M范围内呈线性关系。利用优化后的体系，建立了一种简单、快速的测定水中甲醛的流动分析方法，平均误差小于1%，检出限为1µ/L，化学发光法和生物发光法在化学分析中具有一定的优势(1-7)。Hercules、Seitz和他的同事将酶诱导的反应与灵敏的鲁米诺化学发光(CL)系统相结合，并在流动系统中进行分析，开发了新的CL技术(1、4、8-10)。这些技术非常成功，因为CL具有敏感性、特异性和简单性的独特优势。鲁米诺在碱性溶液中的氧化是最有效、最著名的化学发光反应之一，被广泛用于分析目的(1，4，11-13)。
Formaldehyde and gallic acid oxidized with aqueous alkaline hydrogen peroxide produce relatively strong chemilumi-nescence in the spectral range 560-850 nm (the Trautz-Schorigln reaction). The kinetics of this system have been measured as well as the chemiluminescence spectra and absorption and fluorescence spectra of intermediates and products. The effects of order of reagent addition and variations In oxygen pressure on chemiluminescence have also been determined. Heat generation and variations In dissolved oxygen concentration have been measured as a function of time and order of reagent addition. The results are discussed In terms of the reaction mechanism with particular emphasis on the possible role of singlet molecular oxygen. The effects of reagent concentrations, pH, temperature, rate flow, and Interfering compounds on the maxi-mum of chemiluminescence intensity were measured. Chemiluminescence Intensity is linearly proportional to formaldehyde concentration from 10-7 to 10-2M. Using the optimized system, a simple and rapid flow method for formaldehyde determination in water was developed with a mean error less than 1% and a detection limit of 1 µ/.Chemiluminescent and bioluminescent methods offer certain advantages for chemical analysis (1-7). Hercules, Seitz, and coworkers, combining enzyme-induced reactions with the sensitive luminol chemiluminescence (CL)-system and performing analyses in a flow system have developed new CL techniques (1, 4, 8-10). These techniques are very successful because of CL’s unique advantages of sensitivity, specificity, and simplicity. The oxidation of luminol in basic solution is one of the most efficient, best known CL reactions and is widely used for analytical purposes (1, 4, 11-13).