Effects of nanoparticle size and cell type on high sensitivity cell detection using a localized surface plasmon resonance biosensor
Effects of nanoparticle size and cell type on high sensitivity cell detection using a localized surface plasmon resonance biosensor
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DOI：
10.1016/j.bios.2013.11.075
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发表时间：
2014-05-15
影响因子：
12.6
通讯作者：
Pang, Stella W.
中科院分区：
文献类型：
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作者：
Liu, Fei;Wong, Matthew Man-Kin;Pang, Stella W.
使用局部表面等离子体共振（LSPR）效应来区分玻璃基板上Au纳米颗粒（NP）有序阵列的细胞浓度。将人类衍生的视网膜色素上皮RPE-1细胞具有平坦的身体和较高的汇合度与乳腺癌MCF-7细胞进行了比较。纳米圈光刻用于形成平均直径为500和60 nm的AU NP，以比较细胞检测范围，谐振峰值移位和细胞浓度敏感性。在较大的500 nm au NP上检测到较大的细胞浓度范围，而60 nm au nps（8.56 x 10（3）-1.09 x 10（6）vs. 3.43 x 10（4）-2.73 x 10（5）细胞/ml）。共振峰值变化可以将RPE-1与两个AU NP上的MCF-7细胞区分开。与MCF-7细胞相比，RPE-1细胞始终显示出较大的共振峰值变化，直到检测在较高浓度下饱和为止。对于两种类型的细胞，与60 nm au nps相比，在500 nm上观察到与10（4）-10（6）细胞/mL相似的浓度敏感性。我们的结果表明，可以在较大范围内和低浓度下检测到AU NP上的细胞。可以通过根据不同的细胞特性和浓度改变AU NP的尺寸来实现最佳细胞感测。 （c）2013 Elsevier B.V.保留所有权利。
A localized surface plasmon resonance (LSPR) effect was used to distinguish cell concentration on ordered arrays of Au nanoparticles (NPs) on glass substrates. Human-derived retinal pigment epithelial RPE-1 cells with flatter bodies and higher confluency were compared with breast cancer MCF-7 cells. Nanosphere lithography was used to form Au NPs with average diameters of 500 and 60 nm in order to compare cell detection range, resonance peak shift, and cell concentration sensitivity. A larger cell concentration range was detected on the larger 500 nm Au NPs compared to 60 nm Au NPs (8.56 X 10(3)-1.09 x 10(6) vs. 3.43 x 10(4)-2.73 x 10(5) cells/ml). Resonance peak shift could distinguish RPE-1 from MCF-7 cells on both Au NPs. RPE-1 cells consistently displayed larger resonance peak shifts compared to MCF-7 cells until the detection became saturated at higher concentration. For both types of cells, higher concentration sensitivity in the range of similar to 10(4)-10(6) cells/ml was observed on 500 nm compared to 60 nm Au NPs. Our results show that cells on Au NPs can be detected in a large range and at low concentration. Optimal cell sensing can be achieved by altering the dimensions of Au NPs according to different cell characteristics and concentrations. (C) 2013 Elsevier B.V. All rights reserved.