Metagenomic evidence for h(2) oxidation and h(2) production by serpentinite-hosted subsurface microbial communities.
Metagenomic evidence for h(2) oxidation and h(2) production by serpentinite-hosted subsurface microbial communities.
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DOI：
10.3389/fmicb.2011.00268
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发表时间：
2012
影响因子：
5.2
通讯作者：
Schrenk MO
中科院分区：
文献类型：
--
作者：
Brazelton WJ;Nelson B;Schrenk MO
地球地幔中的超镁铁质岩石代表了巨大的碳和还原力的储存库。在构造抬升和暴露于流体流动时，这些物质的蛇纹岩化作用产生丰富的能量，维持有机分子的非生物合成，并释放氢气（H2）。为了评估潜在的微生物H2利用燃料的蛇纹石化，我们进行了宏基因组调查的海洋蛇纹岩托管热液烟囱（在迷城热液领域）和两个大陆蛇纹岩托管碱性渗漏（在高原蛇绿岩，纽芬兰）。新的[NiFe]-氢化酶序列被确定在海洋和大陆的网站，并在这两种情况下，系统发育分析表明，好氧，潜在的自养Betaproteobacteria属于Burkholderiales作为最有可能的H2-oxidizers。这两个网站也产生了宏基因组证据的微生物H2生产催化[FeFe]-氢化酶在厌氧革兰氏阳性菌属于梭菌目。此外，我们目前的宏基因组证据在这两个网站的有氧一氧化碳利用和厌氧碳固定通过伍德-永达尔途径。在一般情况下，我们的研究结果指出，H2-氧化Betaproteobacteria繁荣浅，缺氧过渡区和厌氧梭菌繁荣缺氧，深地下栖息地。这些数据表明，宏基因组调查的可行性，通过表面暴露的渗漏到新的地下栖息地，并表明在蛇纹岩托管地下栖息地的H2动力的初级生产的潜力。
Ultramafic rocks in the Earth’s mantle represent a tremendous reservoir of carbon and reducing power. Upon tectonic uplift and exposure to fluid flow, serpentinization of these materials generates copious energy, sustains abiogenic synthesis of organic molecules, and releases hydrogen gas (H2). In order to assess the potential for microbial H2 utilization fueled by serpentinization, we conducted metagenomic surveys of a marine serpentinite-hosted hydrothermal chimney (at the Lost City hydrothermal field) and two continental serpentinite-hosted alkaline seeps (at the Tablelands Ophiolite, Newfoundland). Novel [NiFe]-hydrogenase sequences were identified at both the marine and continental sites, and in both cases, phylogenetic analyses indicated aerobic, potentially autotrophic Betaproteobacteria belonging to order Burkholderiales as the most likely H2-oxidizers. Both sites also yielded metagenomic evidence for microbial H2 production catalyzed by [FeFe]-hydrogenases in anaerobic Gram-positive bacteria belonging to order Clostridiales. In addition, we present metagenomic evidence at both sites for aerobic carbon monoxide utilization and anaerobic carbon fixation via the Wood–Ljungdahl pathway. In general, our results point to H2-oxidizing Betaproteobacteria thriving in shallow, oxic–anoxic transition zones and the anaerobic Clostridia thriving in anoxic, deep subsurface habitats. These data demonstrate the feasibility of metagenomic investigations into novel subsurface habitats via surface-exposed seeps and indicate the potential for H2-powered primary production in serpentinite-hosted subsurface habitats.
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DOI：
10.1073/pnas.0600035103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Biddle, JF;Lipp, JS;Hinrichs, KU
通讯作者：
Hinrichs, KU
影响因子：
4.4
作者：
Boyd, Eric S.;Spear, John R.;Peters, John W.
通讯作者：
Peters, John W.
影响因子：
4.4
作者：
Huber, JA;Butterfield, DA;Baross, JA
通讯作者：
Baross, JA
影响因子：
1.2
作者：
Fricke, Wolfgang Florian;Kusian, Bernhard;Bowien, Botho
通讯作者：
Bowien, Botho
影响因子：
4.4
作者：
Imachi, H;Sekiguchi, Y;Harada, H
通讯作者：
Harada, H