A ratiometric fluorescent probe for visualization of thiophenol and its applications
A ratiometric fluorescent probe for visualization of thiophenol and its applications
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苯硫酚可视化比率荧光探针及其应用
DOI：
10.1016/j.saa.2020.118061
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Haitao Li
中科院分区：
文献类型：
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作者：
Youming Shen;Lingcong Dai;Youyu Zhang;Xiangyang Zhang;Chunxiang Zhang;Shaoheng Liu;Yucai Tang;Haitao Li
硫酚在农业和工业中有着广泛的应用。然而，噻吩酚因其高毒性而对环境和健康造成危害。开发一种有效的检测噻吩的方法在环境和生物学领域具有重要的应用价值。本文首次以（E)-7-（二乙基氨基）-3-（2-（二乙基氨基）-2-氧- 2h - chromen3 -基）乙烯基为荧光基团，2,4-二硝基苯基乙烯基）- 2h - chromen2 -one （DCVP）为荧光基团，构建了一种反应型比例荧光探针(E)-4-(2-（二乙基氨基）-2-氧- 2h - chromen1 -溴化铵（DCVP-DNP），用于探测环境和细胞中的噻吩。该探针通过噻吩引发的亲核取代反应对噻吩具有高选择性。在0 ~ 65 μM范围内，探针的发射强度比与噻吩浓度呈线性关系，对噻吩的检出限低至4.8 × 10−8M。此外，该探针不仅可以用于水样中噻吩的检测，还可以对活细胞中的噻吩进行成像，表明其在环境和生物系统中的潜在应用前景。
Thiophenol has a broad application in agriculture and industry. However, thiophenol can harm to the environment and health for its high toxicity. Developing an effective method for detection of thiophenol in the field of environmental and biology is valuable. In this work, we construct a reaction-based ratiometric fluorescent probe (E)-4-(2-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)vinyl)-1-(4-(2,4-dinitrophenoxy)benzyl)pyridin-1-ium bromide (DCVP-DNP) for probing thiophenol in environment and cells by employing (E)-7-(diethylamino)-3-(2-(pyridin-4-yl)vinyl)-2H-chromen-2-one (DCVP) as the fluorophore and 2,4-dinitrophenyl (DNP) ether as the recognition group for the first time. The probe has high selectivity for thiophenol though thiophenol-triggered nucleophilic substitution reaction. In addition, the ratio of emission intensities of the probe has linearly with thiophenol concentration in the range of 0–65 μM and the detection limit of thiophenol is as low as 4.8 × 10−8M. Moreover, the probe can not only be applied for detection of thiophenol in water samples, but also image thiophenol in living cells, suggesting its potential application in environment and biological system.