The WY domain in the Phytophthora effector PSR1 is required for infection and RNA silencing suppression activity
The WY domain in the Phytophthora effector PSR1 is required for infection and RNA silencing suppression activity
复制标题
疫霉效应子 PSR1 中的 WY 结构域是感染和 RNA 沉默抑制活性所必需的
DOI：
10.1111/nph.15836
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发表时间：
2019
期刊：
影响因子：
9.4
通讯作者：
Qiao Yongli
中科院分区：
文献类型：
--
作者：
Zhang Peng;Jia Yijuan;Shi Jinxia;Chen Chen;Ye Wenwu;Wang Yuanchao;Ma Wenbo;Qiao Yongli
疫霉通过分泌大量的RxLR效应物来操纵宿主的先天免疫，从而促进病原菌的定植。预测的WY结构域的单个和串联重复是整个疫霉RxLR超家族中最重要的C-末端基序。然而，单独的WY结构域在效应器中的功能仍然被很少了解。SOJAJEVER疫霉PSR1通过抑制植物寄主中小RNA的生物合成而促进感染。我们在PSR1中发现了一个跟随RxLR基序的单个WY结构域。在烟草、拟南芥和大豆中，该结构域是RNA沉默、抑制活性和侵染所必需的。WY结构域保守残基的突变并不影响PSR1的亚细胞定位，但取消了它对植物发育和对病毒和疫霉病原体的抗性。这至少部分是由于植物中PSR1突变体的蛋白质稳定性降低所致。对PSR1中WY结构域的鉴定允许预测PSR1样效应子家族也具有RNA沉默抑制活性。寄生疫霉PpPSR1L和辣椒疫霉PcPSR1L保守残基的突变干扰了它们的生物学功能，表明WY结构域在疫霉PSR1和PSR1样效应器中起关键作用。
Phytophthorapathogens manipulate host innate immunity by secreting numerous RxLR effectors, thereby facilitating pathogen colonization. Predicted single and tandem repeats of WY domains are the most prominent C‐terminal motifs conserved across thePhytophthoraRxLR superfamily. However, the functions of individual WY domains in effectors remain poorly understood.ThePhytophthora sojaeeffector PSR1 promotes infection by suppressing small RNA biogenesis in plant hosts. We identified one single WY domain following the RxLR motif in PSR1. This domain was required for RNA silencing suppression activity and infection inNicotiana benthamiana,Arabidopsisand soybean.Mutations of the conserved residues in the WY domain did not affect the subcellular localization of PSR1 but abolished its effect on plant development and resistance to viral andPhytophthorapathogens. This is at least in part due to decreased protein stability of the PSR1 mutantsin planta.The identification of the WY domain in PSR1 allows predicts that a family of PSR1‐like effectors also possess RNA silencing suppression activity. Mutation of the conserved residues in two members of this family, PpPSR1L fromP. parasiticaand PcPSR1L fromP. capsici, perturbed their biological functions, indicating that the WY domain is critical inPhytophthoraPSR1 and PSR1‐like effectors.