Fine-scale variation in meiotic recombination in Mimulus inferred from population shotgun sequencing
Fine-scale variation in meiotic recombination in Mimulus inferred from population shotgun sequencing
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DOI：
10.1073/pnas.1319032110
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发表时间：
2013-11-26
影响因子：
11.1
通讯作者：
Rokhsar, Daniel S.
中科院分区：
文献类型：
--
作者：
Hellsten, Uffe;Wright, Kevin M.;Rokhsar, Daniel S.
减数分裂重组率在不同的基因组中差异很大，在寒冷地区，密集活动的热点穿插在一起。在酵母中，热点往往发生在基因的启动子区域，而在人类和小鼠中，热点主要由正调控结构域锌指蛋白9的结合位点定义。为了研究开花植物的详细重组模式，我们对猕猴花（Mimulus guttatus）的野生种群进行了鸟枪重测序，以精确定位超过40万个历史交叉或基因转换区域的边界。它们的分布定义了大约13000个不同强度的热点，穿插着难以察觉的低重组的寒冷地区。平均重组率在基因起始处达到峰值，然后急剧下降，表现出极性。在基因内，重组束更可能终止于外显子而不是内含子。一般模式与在酵母以及正调控结构域锌指蛋白9敲除小鼠中观察到的相似，这表明在Mimulus中描述的重组起始可能反映了古老而保守的真核生物机制。
Meiotic recombination rates can vary widely across genomes, with hotspots of intense activity interspersed among cold regions. In yeast, hotspots tend to occur in promoter regions of genes, whereas in humans and mice, hotspots are largely defined by binding sites of the positive- regulatory domain zinc finger protein 9. To investigate the detailed recombination pattern in a flowering plant, we use shotgun resequencing of a wild population of the monkeyflower Mimulus guttatus to precisely locate over 400,000 boundaries of historic crossovers or gene conversion tracts. Their distribution defines some 13,000 hotspots of varying strengths, interspersed with cold regions of undetectably low recombination. Average recombination rates peak near starts of genes and fall off sharply, exhibiting polarity. Within genes, recombination tracts are more likely to terminate in exons than in introns. The general pattern is similar to that observed in yeast, as well as in positiveregulatory domain zinc finger protein 9- knockout mice, suggesting that recombination initiation described here in Mimulus may reflect ancient and conserved eukaryotic mechanisms.