THE INTERPLAY OF DNA-BINDING PROTEINS ON THE PROMOTER OF THE MOUSE ALBUMIN GENE
THE INTERPLAY OF DNA-BINDING PROTEINS ON THE PROMOTER OF THE MOUSE ALBUMIN GENE
复制标题
DOI：
10.1016/0092-8674(87)90583-6
复制
发表时间：
1987-12-24
期刊：
影响因子：
64.5
通讯作者：
SCHIBLER, U
中科院分区：
文献类型：
--
作者：
LICHTSTEINER, S;WUARIN, J;SCHIBLER, U
小鼠白蛋白基因的启动子包含至少六个特定dna结合蛋白的结合位点（A到F）。其中四个位点（A、D、E和F）可以被转录因子占据，这些转录因子在肝核中比在脾核或脑核中丰富得多。这些因子包括一种热稳定的蛋白质，填充a、D和F位点，以及一种核因子I （NF-I）相关蛋白质家族的成员，占据e位点。位点C结合一种在肝、脑和脾核中同样丰富的蛋白质。这个位置的占用和热稳定因子与紧邻的位置D的结合似乎是相互排斥的。然而，这两个相互竞争的结合位点都是体外转录最大化所必需的。
The promoter of the mouse albumin gene contains at least six binding sites for specific DNA-binding proteins (A to F). Four of these sites (A, D, E, and F) can be occupied by transcription factors that are considerably enriched in liver nuclei, as compared to spleen or brain nuclei. These factors consist of a heat-stable protein that fills sites A, D, and F, and a member of a family of nuclear factor I (NF-I) related proteins that occupies site E. Site C binds a protein that is equally abundant in liver, brain, and spleen nuclei. Occupancy of this site and the binding of the heat-stable factor to the immediately adjacent site D appear to be mutually exclusive. However, both of these competing binding sites are required for maximal in vitro transcription.