Shoot organogenesis and somatic embryogenesis from leaf explants of Valeriana jatamansi Jones
Shoot organogenesis and somatic embryogenesis from leaf explants of Valeriana jatamansi Jones
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缬草叶外植体的芽器官发生和体细胞胚胎发生
DOI：
10.1016/j.scienta.2013.11.036
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发表时间：
2014-01-22
影响因子：
4.3
通讯作者：
Ma, Guohua
中科院分区：
文献类型：
--
作者：
Chen, Rong;Zhang, Muhan;Ma, Guohua
以蜘蛛香离体叶片为外植体，从胚性愈伤组织诱导不定芽和体细胞胚。这两种形态发生过程可以诱导Murashige和Skoog（MS）的基本培养基上添加不同浓度和组合的植物生长调节剂。胚性愈伤组织仅在生长素即1.0-7.5 μ M的2，4-二氯苯氧乙酸（2，4-D）和5.0 μ M的α-萘乙酸（NAA）存在下诱导，当留在同一培养基上时，分别分化成不定芽和体细胞胚。愈伤组织在附加5.0 μ M吲哚丁酸（IBA）、5.0 μ M NAA或1.0-5.0 μ M 2，4 -0的MS培养基上诱导不定根。细胞分裂素单独添加到愈伤组织诱导培养基中，包括6-苄基腺嘌呤、激动素和噻苯隆，在5.0 μ M时不能诱导愈伤组织，除非与0.25-1.0 μ M NAA组合。(C)2013爱思唯尔有限公司版权所有。
The in vitro leaves of Valeriana jatamansi Jones were used as explants to induce adventitious shoots and somatic embryos from embryogenic callus. Both morphogenic processes could be induced on Murashige and Skoog (MS) basal medium supplemented with different concentrations and combinations of plant growth regulators. Embryogenic callus, which was induced exclusively in the presence of auxins, namely 1.0-7.5 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) and 5.0 mu M alpha-naphthaleneacetic acid (NAA), differentiated into adventitious shoots and somatic embryos, respectively when left on the same medium. Callus, when placed on MS medium containing 5.0 mu M indole-3-butyric acid (IBA), 5.0 mu M NAA or 1.0-5.0 mu M 2,4-0, induced adventitious roots. Cytokinins that were added singly to callus induction medium, including 6-benzyladenine, kinetin and thidiazuron, could not induce callus at 5.0 mu M except when combined with 0.25-1.0 mu M NAA. (C) 2013 Elsevier B.V. All rights reserved.