Ischemic stroke induces gut permeability and enhances bacterial translocation leading to sepsis in aged mice.
Ischemic stroke induces gut permeability and enhances bacterial translocation leading to sepsis in aged mice.
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DOI：
10.18632/aging.100952
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
McCullough LD
中科院分区：
文献类型：
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作者：
Crapser J;Ritzel R;Verma R;Venna VR;Liu F;Chauhan A;Koellhoffer E;Patel A;Ricker A;Maas K;Graf J;McCullough LD
老龄化是卒中后感染的重要危险因素，占卒中相关死亡率的很大比例。尽管如此，在老年动物模型中评估中风后感染率的研究是有限的。此外，很少有研究评估肠道微生物是中风后感染的潜在来源。因此，我们研究了年龄的影响和肠道细菌移位在年轻（8-12周）和老年（18-20个月）C57 Bl/6雄性小鼠短暂大脑中动脉闭塞（MCAO）或假手术后中风后感染中的作用。检查肠通透性，并评估中风后外周器官中肠源性细菌的存在。此外，疾病参数和先天性和适应性免疫的组成部分进行了检查。我们发现，虽然中风在年轻和老年小鼠中都诱导了肠道通透性和细菌移位，但只有年轻小鼠能够解决感染。接种外周器官的细菌种类在年轻小鼠（埃希氏菌属）和老年小鼠（肠杆菌属）之间也不同。因此，与中风后的年轻小鼠相比，老年小鼠出现了以持续和加重的体温过低、体重减轻和免疫功能障碍为标志的脓毒性反应。
Aging is an important risk factor for post-stroke infection, which accounts for a large proportion of stroke-associated mortality. Despite this, studies evaluating post-stroke infection rates in aged animal models are limited. In addition, few studies have assessed gut microbes as a potential source of infection following stroke. Therefore we investigated the effects of age and the role of bacterial translocation from the gut in post-stroke infection in young (8-12 weeks) and aged (18-20 months) C57Bl/6 male mice following transient middle cerebral artery occlusion (MCAO) or sham surgery. Gut permeability was examined and peripheral organs were assessed for the presence of gut-derived bacteria following stroke. Furthermore, sickness parameters and components of innate and adaptive immunity were examined. We found that while stroke induced gut permeability and bacterial translocation in both young and aged mice, only young mice were able to resolve infection. Bacterial species seeding peripheral organs also differed between young (Escherichia) and aged (Enterobacter) mice. Consequently, aged mice developed a septic response marked by persistent and exacerbated hypothermia, weight loss, and immune dysfunction compared to young mice following stroke.