Effect of vapors from fractionated samples of propolis on microbial and oxidation damage of rice during storage

Effect of vapors from fractionated samples of propolis on microbial and oxidation damage of rice during storage
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DOI:
10.1016/j.jfoodeng.2008.02.019
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发表时间:
2008-10
影响因子:
5.5
通讯作者:
G. Griffiths;Uchino Toshitaka;Tanaka Fumihiko;Hamanaka Daisuke
G. Griffiths;Uchino Toshitaka;Tanaka Fumihiko;Hamanaka Daisuke
中科院分区:
农林科学1区
文献类型:
--
作者:
G. Griffiths;Uchino Toshitaka;Tanaka Fumihiko;Hamanaka Daisuke

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研究了水稻挥发油极性和非极性亚组分的挥发油对水稻生长过程中微生物和氧化的控制效果。储存进行了评估。将亚组分(无水乙醇、二氯甲烷、己烷浸提液:分别为AEPEV、MCPEV和HEPEV)注入合成吸附剂中,并在储存期间(6个月)释放挥发物。HEPEV、MCPEV和AEPEV处理抑制糙米上的霉变和接种后细菌定殖(分别减少1.1、1.1、0.9和1.3、1.2、1.1log 10 cfu/g)。AEPEV处理抑制了30 ° C下精米的脂肪酸损伤至常规冷藏水平(5 ° C),并且处理后分离的细菌的差异革兰氏染色表明占优势的革兰氏阳性细菌分布。正己烷(HEPE)、无水乙醇(AEPE)和二氯甲烷(MCPE)提取物清除2 ′,2 ′-二苯基苦肼基自由基的50%抑制浓度分别为9.8、3.2和2.8 μ g/μ l。在β-胡萝卜素漂白试验中,AEPE的氧化降解速率最低(4.3 × 10 − 4min − 1),HEPE的氧化降解速率最高(1.9 × 10 − 3min − 1)。气相色谱质谱分析表明,AEPE具有最高量的咖啡酸和咖啡酸苯乙酯。各亚组分的挥发性物质在稻米品质保持方面具有不同的潜力。
The efficacy of vapors from polar and non-polar sub-fractions of propolis on microbial and oxidation control during rice (Oryza sativa, hinohikari var.) storage was evaluated. The sub-fractions (absolute ethanol, methylene chloride, hexane extracts: AEPEV, MCPEV and HEPEV, respectively) were infused in synthetic adsorbents and their volatiles released during storage (6 months). HEPEV, MCPEV and AEPEV treatments inhibited molding and post-inoculation bacterial colonization (1.1, 1.1, 0.9 and 1.3, 1.2, 1.1log10cfu/g reductions, respectively) on brown rice. AEPEV treatment suppressed fat acidity damage of milled rice at 30°C to conventional cold storage level (5°C) and differential Gram staining of bacteria isolated after the treatment indicated a dominant Gram-positive bacterial distribution. The concentrations providing 50% inhibition of 2′,2′-diphenylpicrylhydrazyl free radical scavenging were 9.8, 3.2 and 2.8μg/μl for hexane (HEPE), absolute ethanol (AEPE) and methylene chloride (MCPE) extracts, respectively. The oxidative degradation rate was lowest for AEPE (4.3×10−4min−1) and highest for HEPE (1.9×10−3min−1) in the β-carotene bleaching assay. Gas chromatograph mass spectrometry revealed that AEPE had the highest amount of caffeic acid and caffeic acid phenethyl ester. Ultimately, the volatiles from the propolis sub-fractions had varied potential in rice quality preservation.