Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability.
Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability.
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DOI：
10.1021/acsami.6b07362
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发表时间：
2016-10-12
影响因子：
9.5
通讯作者：
Singamaneni S
中科院分区：
文献类型：
--
作者：
Wang C;Luan J;Tadepalli S;Liu KK;Morrissey JJ;Kharasch ED;Naik RR;Singamaneni S
基于局域表面等离子体共振的等离子体生物传感器由于其高灵敏度、成本效益和作为即时生物诊断设备的巨大潜力而获得了极大的关注。然而，大多数等离子体生物传感器和常规生物测定依赖于天然抗体，其对升高的温度和非水性介质敏感。因此，昂贵且笨重的“冷链”系统是必要的，以通过在运输、储存和处理期间维持最佳低温来保存不稳定抗体。在此，我们介绍了一种简便的方法，即使在升高的温度下，也可以保持生物传感器表面上的抗体活性。我们表明丝素蛋白膜可用作保护层，以在室温和40 °C下保持模型抗体（兔IgG）和心肌肌钙蛋白抗体的活性数天。此外，简单的水冲洗过程恢复了生物传感器的生物功能。这种节能环保的方法代表了一种新的方法，以消除诊断试剂和材料的冷链和温度控制包装，从而将基于抗体的生物传感器的能力扩展到不同的资源有限的情况下，如发展中国家，救护车，重症监护室急诊室和战场。
Because of their high sensitivity, cost-efficiency, and great potential as point-of-care biodiagnostic devices, plasmonic biosensors based on localized surface plasmon resonance have gained immense attention. However, most plasmonic biosensors and conventional bioassays rely on natural antibodies, which are susceptible to elevated temperatures and nonaqueous media. Hence, an expensive and cumbersome “cold chain” system is necessary to preserve the labile antibodies by maintaining optimal cold temperatures during transport, storage, and handling. Herein, we introduce a facile approach to preserve the antibody activity on a biosensor surface even at elevated temperatures. We show that silk fibroin film could be used as a protective layer to preserve the activity of a model antibody (Rabbit IgG) and cardiac troponin antibody at both room temperature and 40 °C over several days. Furthermore, a simple aqueous rinsing process restores the biofunctionality of the biosensor. This energy-efficient and environmentally friendly method represents a novel approach to eliminate the cold chain and temperature-controlled packing of diagnostic reagents and materials, thereby extending the capability of antibody-based biosensors to different resource-limited circumstances such as developing countries, an ambulance, an intensive care unit emergency room, and battlefield.