Differentiation capacity of endothelial progenitor cells correlates with endothelial function in healthy young men.
Differentiation capacity of endothelial progenitor cells correlates with endothelial function in healthy young men.
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DOI：
10.1253/circj.cj-08-0952
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发表时间：
2009-06
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影响因子：
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通讯作者：
Manabu Miura;Y. Numaguchi;M. Ishii;R. Kubota;Toyonari Takeuchi;A. Imamura;Ryuichiro Murakami;T. Kondo;K. Okumura;T. Murohara
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文献类型：
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作者：
Manabu Miura;Y. Numaguchi;M. Ishii;R. Kubota;Toyonari Takeuchi;A. Imamura;Ryuichiro Murakami;T. Kondo;K. Okumura;T. Murohara
背景内皮祖细胞（EPCs）被认为是维持血管内皮完整性的细胞，因此本研究调查了EPCs的功能能力是否与健康年轻受试者的内皮功能相关，正如在患有动脉粥样硬化疾病的老年受试者中所证实的那样。方法和结果：对41例健康、年轻男性非吸烟者（年龄33.1 ± 3.9岁，平均值± SD）的EPCs进行了表征。血流介导的血管舒张（FMD）与EPCs数量或生长因子（如血管内皮生长因子）血浆浓度之间的相关性未达到统计学显著性。FMD与EPC分化指数（EPC数占贴壁细胞总数的比值）和内皮型一氧化氮合酶mRNA丰度（r= 0.340，P =0.030）显著相关（r= 0.391，P= 0.011）。结论：在健康的年轻男性中，尽管EPCs的数量或集落计数缺乏相关性，但循环祖细胞分化为内皮细胞系的能力与内皮功能密切相关。该细胞功能测定可作为动脉粥样硬化前阶段健康受试者血管功能的新生物标志物。
BACKGROUND Endothelial progenitor cells (EPCs) have been assumed to maintain vascular endothelial integrity, so the present study investigated whether the functional capacity of EPCs correlates with endothelial function in healthy young subjects, as has been confirmed in aged subjects with atherosclerotic disease. METHODS AND RESULTS EPCs in 41 healthy, young male nonsmokers (age 33.1 +/-3.9 years, mean +/- SD) were characterized. The correlation between flow-mediated vasodilation (FMD) and the number of EPCs or the plasma concentrations of growth factors, such as vascular endothelial growth factor, did not reach statistical significance. However, FMD was significantly correlated with the EPC differentiation index, defined as the ratio of the number of EPCs to the total number of adherent cells (r=0.391, P=0.011) and the abundance of endothelial nitric oxide synthase mRNA (r=0.340, P=0.030). CONCLUSIONS In healthy young men, despite a lack of correlation of the number or colony counts of EPCs, the ability of circulating progenitor cells to differentiate into an endothelial lineage is closely correlated with endothelial function. This cell function assay may serve as a novel biomarker for vascular function in healthy subjects in the pre-atherosclerotic stage.