Reverse genetics of coronaviruses using vaccinia virus vectors.
Reverse genetics of coronaviruses using vaccinia virus vectors.
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DOI：
10.1007/3-540-26765-4_7
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发表时间：
2005
影响因子：
--
通讯作者：
Siddell SG
中科院分区：
文献类型：
--
作者：
Thiel V;Siddell SG
In this article, we describe the reverse genetic system that is based on the use of vaccinia virus cloning vectors. This system represents a generic approach to coronavirus reverse genetics and was first described for the generation of recombinant human coronavirus 229E representing a group I coronavirus. Subsequently, the same approach has been used to generate recombinant avian infectious bronchitis coronavirus and, recently, recombinant mouse hepatitis virus, representing group III and group II coronaviruses, respectively. We describe how vaccinia virus-mediated homologous recombination can be used to introduce specific mutations into the coronavirus genomic cDNA during its propagation in vaccinia virus and how recombinant coronaviruses can be isolated. Finally, we describe how the coronavirus reverse genetic system has now been extended to the generation of coronavirus replicon RNAs.
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影响因子：
5.4
作者：
Herold, J;Gorbalenya, AE;Siddell, SG
通讯作者：
Siddell, SG
影响因子：
5.4
作者：
Curtis, KM;Yount, B;Baric, RS
通讯作者：
Baric, RS
影响因子：
6
作者：
Summers, KL;Hock, BD;Hart, DNJ
通讯作者：
Hart, DNJ
影响因子：
5.4
作者：
Thiel, V;Karl, N;Siddell, SG
通讯作者：
Siddell, SG
DOI：
10.1074/jbc.m104097200
发表时间：
2001-08-31
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ziebuhr J;Thiel V;Gorbalenya AE
通讯作者：
Gorbalenya AE