High-throughput chemiluminescence immunoassay based on Co2+/hemin synergistic catalysis for sensitive detection tetrabromobisphenol A bis(2-hydroxyethyl) ether in the environments
High-throughput chemiluminescence immunoassay based on Co2+/hemin synergistic catalysis for sensitive detection tetrabromobisphenol A bis(2-hydroxyethyl) ether in the environments
复制标题
基于Co2/氯化血红素协同催化的高通量化学发光免疫分析法灵敏检测环境中的四溴双酚A双(2-羟乙基)醚
DOI：
10.1016/j.scitotenv.2020.136880
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发表时间：
2020
影响因子：
9.8
通讯作者：
Zhang Zhen
中科院分区：
文献类型：
--
作者：
Gu Lantian;Zou Yanmin;Li Yanshen;Zeng Kun;Zhu Nuanfei;Zhu Fang;Gyima Eric;Yakub Salome;Meng Hui;Zhang Zhen
在此，制备了一种新型化学发光（CL）免疫测定法，用于灵敏测定四溴双酚 A 双（2-羟乙基）醚（TBBPA-DHEE），这是一种典型的四溴双酚 A 衍生物。在间接竞争法中，合成的PS@hemin@Co2+被二抗（Ab2）代替普通天然酶标记，对鲁米诺-H2O2分解产生CL信号表现出优异的催化作用。此外，由于检测系统中氯高铁血红素和Co2+的协同催化作用，CL信号被大大放大。在优化条件下，所建立的方法具有低检测限（LOD，0.9 μg/L），比使用相同抗体的常规 ELISA 低近 5 倍； (ii) 良好的线性 (1.6–14.3 µg/L)； (iii) 令人满意的准确度和精密度（回收率，89.67–125.33%；CV，2.75–8.37%）。所提出的化学发光免疫测定法用于分析从中国江苏省和浙江省收集的各种来源的环境样品。环境水中的检测浓度范围为 2.4–3.7 µg/L，土壤样品中的检测浓度范围为 1.8–2.4 ng/g（干重，dw），表明从环境样品中检测痕量 TBBPA-DHEE 的巨大潜力。
Here, a novel chemiluminescence (CL) immunoassay was fabricated for sensitive determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE), one of typical tetrabromobisphenol A derivatives. At the indirectly competitive method, the synthesized PS@hemin@Co2+was labelled by secondary antibody (Ab2) instead of common natural enzymes, which showed excellent catalysis towards the decomposition of luminol-H2O2for producing CL signal. Furthermore, the CL signal was greatly amplified owing to the synergistic catalysis of hemin and Co2+in the detection system. Under the optimized conditions, the established method offered (i) low detection limit (LOD, 0.9 μg/L), which was almost 5 times lower than that using a conventional ELISA with the same antibody; (ii) a good linearity (1.6–14.3 μg/L); (iii) satisfactory accuracy and precision (recoveries, 89.67–125.33%; CV, 2.75–8.37%). The proposed CL immunoassay was applied for analysis of environmental samples from various sources collected from Jiangsu and Zhejiang province, China. And the detected concentrations were ranged in 2.4–3.7 μg/L in environmental waters and 1.8–2.4 ng/g (dry weight, dw) in soil samples, indicating great potential for trace TBBPA-DHEE detection from environmental samples.