The effect of phytic acid on the resolution of peptides and proteins in capillary electrophoresis.
The effect of phytic acid on the resolution of peptides and proteins in capillary electrophoresis.
复制标题
植酸对毛细管电泳中肽和蛋白质分辨率的影响。
DOI：
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发表时间：
1994
影响因子：
2.9
通讯作者：
P. Camilleri
中科院分区：
文献类型：
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作者：
G. Okafo;H. Birrell;M. Greenaway;M. Haran;P. Camilleri
研究了植酸钠盐对多肽和蛋白质拆分的影响。在肽的情况下，改进的分离显示是由于带正电荷的肽和植酸聚阴离子物种之间的离子-离子配对相互作用。与蛋白质相关的改进的峰形可以解释为由于将分析物从水介质注射到高离子强度的样品预浓缩。
Studies are reported on the effect of the sodium salt of phytic acid on the resolution of peptides and proteins. Improved separation in the case of peptides is shown to be due to ion-ion pairing interactions between the positively charged peptides and the phytic acid polyanionic species. The improved peak shapes related to the proteins can be interpreted in terms of the sample preconcentration due to injection of analytes from a water medium to one of high ionic strength.