A simple, one-pot and ultrasensitive DNA sensor via Exo III-Assisted target recycling and 3D DNA walker cascade amplification
A simple, one-pot and ultrasensitive DNA sensor via Exo III-Assisted target recycling and 3D DNA walker cascade amplification
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一种简单、一锅式、超灵敏的 DNA 传感器，通过 Exo III 辅助靶标回收和 3D DNA walker 级联放大
DOI：
10.1016/j.aca.2020.12.026
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发表时间：
2021
影响因子：
6.2
通讯作者：
Wang Jian-Hua
中科院分区：
文献类型：
--
作者：
Wu Na;Wang Yi-Ting;Wang Xiao-Yan;Chen Xu-Wei;Yang Ting;Wang Jian-Hua
快速、灵敏和用户友好的核酸检测在早期临床诊断中越来越重要。本研究通过核酸外切酶III（Exo III）辅助的靶循环扩增（ERA）结合三维DNA步行者级联扩增技术构建了一种简单、一锅法、超灵敏的DNA传感器。在单链DNA靶存在的情况下，ERA过程被激活以产生许多步行者链（WS）。此后，Exo III供电的WS自主地沿着沿着基于磁珠（MB）的3D轨道移动，以将大量AgNC释放到上清液中作为放大的信号输出。该生物传感器具有18 fM的低检测限和40 fM至1 pM的分析范围。此外，该生物传感器的实际应用潜力也证实了对p53的人血清和尿液样品的加标实验。
Rapid, sensitive, and user-friendly nucleic acid detection is of growing importance in early clinical diagnosis. Here, we construct a simple, one-pot and ultrasensitive DNA sensor via exonuclease III (Exo III)-assisted target recycling amplification (ERA) combined with 3D DNA walker cascade amplification. In the presence of single-stranded DNA target, the ERA process is activated to generate numerous walker strands (WS). Thereafter, Exo III-powered WSs autonomously move along magnetic bead (MB)-based 3D track to release numerous AgNCs into the supernatant as an amplified signal output. This biosensor had a low detection limit of 18 fM and an analytical range of 40 fM to 1 pM. Furthermore, the practical application potential of this biosensor was also confirmed by the spiking experiments of p53 into human serum and urine samples.