Patients with Primary Sjogren's Syndrome Have Alterations in Absolute Quantities of Specific Peripheral Leucocyte Populations
Patients with Primary Sjogren's Syndrome Have Alterations in Absolute Quantities of Specific Peripheral Leucocyte Populations
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DOI：
10.1111/sji.12622
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发表时间：
2017-12-01
影响因子：
3.7
通讯作者：
Appel, S.
中科院分区：
文献类型：
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作者：
Davies, R.;Hammenfors, D.;Appel, S.
原发性干燥综合征（pSS）缺乏外周血计数的准确解剖。本研究的目的是定量pSS患者外周血中不同的白细胞群。在86例pSS患者和74例健康供体中，通过流式细胞术定量21种不同亚型的特定白细胞亚群的数量。pSS患者的亚组根据腺外表现（EGM）和SSA/SSB自身抗体的存在进行分层。总体而言，pSS患者表现出减少的淋巴细胞亚群相比，健康的捐助者。这种下降在SSA/SSB阳性患者和EGM患者中更为明显。与健康供体相比，pSS患者的粒细胞和单核细胞亚群增加，SSA/SSB阳性患者增加最多。使用基于细胞数量的无监督分层聚类将pSS患者进一步分为四个聚类。以较高浓度的NKT细胞、CD 56 hi NK细胞、CD 20（+）CD 38（-）B细胞和CD 8（+）CD 38（-）T细胞为特征的其中一个簇与比其他簇更弱的临床症状相关，可能标志着较轻的疾病表型。总之，我们的分析表明pSS患者外周血白细胞的细胞谱发生了显著变化，这可能有助于根据疾病严重程度对患者进行分层。
An accurate dissection of peripheral blood enumeration is lacking in primary Sjogren's syndrome (pSS). The purpose of this study was to quantify different leucocyte populations in peripheral blood of patients with pSS. Numbers of specific leucocyte subsets were determined in 86 pSS patients and 74 healthy donors quantifying 21 distinct subtypes by flow cytometry. Subgroups of pSS patients were stratified based on presence of extraglandular manifestations (EGMs) and SSA/SSB autoantibodies. Overall, pSS patients manifested decreased lymphocyte subpopulations compared to healthy donors. Such decreases were more pronounced in SSA/SSB positive patients and patients with EGM. Granulocyte and monocyte subpopulations were increased in pSS patients compared to healthy donors, with the greatest increases in SSA/SSB positive patients. Unsupervised hierarchal clustering based on cell quantities was used to further subgroup the pSS patients into four clusters. One of the clusters characterized by higher concentrations of NKT cells, CD56hi NK cells, CD20(+)CD38(-) B cells and CD8(+)CD38(-) T cells was associated with weaker clinical symptoms than the other clusters, possibly marking a milder disease phenotype. In conclusion, our analyses indicate significant alterations in the cellular profiles of peripheral blood leucocytes in patients with pSS and may help to stratify the patients according to disease severity.